Ouariagli Imane, Alaoui Sosse Soukayna, Laraqui Abdelilah, Tagajdid Mohammed Reda, Mrabti Mohammed, Alami Mohamed, Ennaji Moulay Mustapha
Laboratory of Virology, Oncology, Biosciences, Environment and New Energies, Faculty of Sciences and Techniques-Mohammedia, University of Hassan II Casablanca, Mohammedia, MAR.
Department of Urology, Mohammed V Military Teaching Hospital, University of Mohammed V Rabat, Rabat, MAR.
Cureus. 2024 Jul 10;16(7):e64275. doi: 10.7759/cureus.64275. eCollection 2024 Jul.
Prostate cancer is one of the most common major health problems. Several risk factors are potentially involved in its development. Therefore, a biomarker capable of early diagnosis is necessary to facilitate the early detection and treatment of prostate cancer. Human endogenous retroviruses (HERVs) are abnormally expressed in various diseases. Our study aims to evaluate the specific role of HERV K-10 gag expressions in the progression of prostate cancer. For this, we collected a set of 50 prostate tumor tissue samples as well as 50 healthy tissue samples. After extracting RNA from the prostate samples, we analyzed the expression of HERV-K gag using quantitative real-time PCR (qRT-PCR). The resulting data revealed a significant correlation of HERV-K gag expression in malignant regions of the prostate in men with prostate cancer than in men without prostate cancer (p < 0.05). The presence of the HERV-K gag protein was detected in 10 of 50 tumor samples (20%), while no healthy samples presented this protein. These results suggest that increased HERV-K gag RNA and protein expression could serve as a sensitive and specific biomarker of prostate malignancy in this cohort of prostate carcinoma patients, further supporting its potential as a promising clinical marker.
前列腺癌是最常见的主要健康问题之一。其发展可能涉及多种风险因素。因此，一种能够早期诊断的生物标志物对于促进前列腺癌的早期检测和治疗至关重要。人类内源性逆转录病毒（HERVs）在多种疾病中异常表达。我们的研究旨在评估HERV K-10 gag表达在前列腺癌进展中的具体作用。为此，我们收集了50份前列腺肿瘤组织样本以及50份健康组织样本。从前列腺样本中提取RNA后，我们使用定量实时PCR（qRT-PCR）分析了HERV-K gag的表达。所得数据显示，前列腺癌男性患者前列腺恶性区域中HERV-K gag的表达与无前列腺癌男性相比存在显著相关性（p < 0.05）。在50个肿瘤样本中有10个（20%）检测到HERV-K gag蛋白的存在，而健康样本中均未出现该蛋白。这些结果表明，HERV-K gag RNA和蛋白表达的增加可作为该组前列腺癌患者前列腺恶性肿瘤的敏感且特异的生物标志物，进一步支持了其作为有前景的临床标志物的潜力。
