Team of Virology, Oncology and Biotechnologies. laboratory of Virology, Oncology, Biosciences, Environment and New Energies, Faculty of Sciences and Techniques- Mohammedia, University Hassan II of Casablanca, Casablanca, Morocco.
Department of urology, Mohammed V Military Teaching Hospital, university of Mohammed V, Rabat, Morocco.
Mol Biol Rep. 2023 Jul;50(7):5719-5724. doi: 10.1007/s11033-023-08474-6. Epub 2023 May 22.
Prostate cancer (PCa) is one of the most common tumors in men, regardless of ethnicity and demographics. In many risk factors causing PCa, genes and viral infections are strong candidates for the development of prostate tumors. Indeed, tissue infections of PCa have been reported by the presence of several types of viruses including Human Papillomaviruses (HPV).
the present study was planned to determine whether HPV DNA could be detected in the blood of known men with prostate cancer and to assess the potential association between HPV infection and clinico-pathological characteristics of the patients.
In order to achieve our objectives, 150 liquid blood samples were taken from Moroccan patients, 100 patients with PCa, and 50 control cases. The viral DNA was extracted, calibrated and the target genes were amplified by PCR using specific primers and the use of 2% agarose gel with visualization under UV.
Of the 100 samples tested, (10%) were infected with HPV), However, none of the control cases were infected with HPV. The analysis of the data made it possible to establish a correlation between the frequency of the viral infection of the human papilloma and the tumoral criteria.
Therefore, this study strengthens the potential role of HPV as a cofactor in prostate cancer development, and we propose that infection with this virus may be involved in the development of PCa metastases.
前列腺癌（PCa）是男性最常见的肿瘤之一，无论种族和人口统计学如何。在导致 PCa 的许多危险因素中，基因和病毒感染是前列腺肿瘤发展的强有力候选者。事实上，已经报道了 PCa 组织感染存在包括人乳头瘤病毒（HPV）在内的多种类型的病毒。
本研究旨在确定已知患有前列腺癌的男性血液中是否可以检测到 HPV DNA，并评估 HPV 感染与患者临床病理特征之间的潜在关联。
为了实现我们的目标，从摩洛哥患者中采集了 150 份液体血液样本，100 份前列腺癌患者和 50 份对照病例。使用特定引物通过 PCR 提取、校准和扩增病毒 DNA，并使用 2%琼脂糖凝胶在紫外线下可视化。
在测试的 100 个样本中，有 10%（10%）感染了 HPV），然而，对照组均未感染 HPV。数据分析建立了人乳头瘤病毒感染的频率与肿瘤标准之间的相关性。
因此，这项研究加强了 HPV 作为前列腺癌发展的协同因素的潜在作用，我们提出感染这种病毒可能参与了 PCa 转移的发展。
