Megquier Kate, Husted Christopher, Rhoades Justin, White Michelle E, Genereux Diane P, Chen Frances L, Xiong Kan, Kwon Euijin, Swofford Ross, Painter Corrie, Adalsteinsson Viktor, London Cheryl A, Gardner Heather L, Karlsson Elinor K
Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Genomics and Computational Biology, UMass Chan Medical School, Worcester, MA, USA.
bioRxiv. 2024 Jul 30:2024.07.29.605605. doi: 10.1101/2024.07.29.605605.
While liquid biopsy has potential to transform cancer diagnostics through minimally-invasive detection and monitoring of tumors, the impact of preanalytical factors such as the timing and anatomical location of blood draw is not well understood. To address this gap, we leveraged pet dogs with spontaneous cancer as a model system, as their compressed disease timeline facilitates rapid diagnostic benchmarking. Key liquid biopsy metrics from dogs were consistent with existing reports from human patients. The tumor content of samples was higher from venipuncture sites closer to the tumor and from a central vein. Metrics also differed between lymphoma and non-hematopoietic cancers, urging cancer-type-specific interpretation. Liquid biopsy was highly sensitive to disease status, with changes identified soon after post chemotherapy administration, and trends of increased tumor fraction and other metrics observed prior to clinical relapse in dogs with lymphoma or osteosarcoma. These data support the utility of pet dogs with cancer as a relevant system for advancing liquid biopsy platforms.
虽然液体活检有潜力通过对肿瘤进行微创检测和监测来改变癌症诊断，但诸如采血时间和解剖位置等分析前因素的影响尚未得到充分了解。为了填补这一空白，我们利用患有自发性癌症的宠物狗作为模型系统，因为它们缩短的疾病进程便于快速进行诊断基准测试。狗的关键液体活检指标与人类患者的现有报告一致。靠近肿瘤的静脉穿刺部位和中心静脉采集的样本肿瘤含量更高。淋巴瘤和非造血系统癌症的指标也有所不同，这就需要针对癌症类型进行解读。液体活检对疾病状态高度敏感，化疗给药后不久就能发现变化，并且在淋巴瘤或骨肉瘤犬临床复发之前观察到肿瘤分数和其他指标增加的趋势。这些数据支持了患有癌症的宠物狗作为推进液体活检平台的相关系统的实用性。
