Keshan Branch of Heilongjiang Academy of Agricultural Sciences, Qiqihaer, Heilongjiang, China.
Key Laboratory of Potato Biology and Genetics, Ministry of Agriculture and Rural Affairs, Qiqihaer, Heilongjiang, China.
PeerJ. 2024 Aug 6;12:e17831. doi: 10.7717/peerj.17831. eCollection 2024.
Browning is a common problem that occurs during potato processing; it is typically resolved by adding chemicals during the production process. However, there is a need to develop potato varieties that are resistant to browning due to a growing consumer interest in healthier diets. This study initially identified 275 potato varieties that are resistant to browning; these were narrowed down to eight varieties, with four of them being highly resistant. A hybrid population was developed by crossing the highly resistant CIP395109.29 with the easily browned Kexin 23. Bulked segregant analysis (BSA) was conducted, which identified 21 potato genes associated with anti-browning properties through sequencing data analysis and organization. The findings of this study lay a solid groundwork for future research on breeding potatoes with anti-browning traits, offer molecular markers for identifying anti-browning varieties, and serve as a valuable reference for further investigations into potato browning mechanisms.
褐变是马铃薯加工过程中常见的问题，通常通过在生产过程中添加化学物质来解决。然而，由于消费者对更健康饮食的兴趣日益增加，因此需要开发出抗褐变的马铃薯品种。本研究最初鉴定出 275 种抗褐变的马铃薯品种；将这些品种缩小到 8 个品种，其中 4 个品种具有高度抗性。通过将高度抗性的 CIP395109.29 与易褐变的克新 23 杂交，开发了一个杂种群体。通过测序数据分析和组织，进行了 bulked segregant analysis（BSA），鉴定出与抗褐变特性相关的 21 个马铃薯基因。本研究为未来具有抗褐变特性的马铃薯育种研究奠定了坚实的基础，为鉴定抗褐变品种提供了分子标记，并为进一步研究马铃薯褐变机制提供了有价值的参考。
