State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Genes (Basel). 2021 Sep 23;12(10):1478. doi: 10.3390/genes12101478.
Trichome is a natural physical barrier protecting plants against environmental stresses, natural infestations, ultraviolet rays and pathogenicity. Trichome also helps plants in maintaining appropriate water content by reducing transpiration rate. The molecular mechanism regulating unicellular trichome development in Arabidopsis has been extensively elucidated, but the molecular mechanism regulating multicellular trichome development remains unclear. In this study, we identified a () mutant from a cucumber EMS (Ethylmethylsulfone) mutagenesis population. Genetic analysis indicated that an incomplete dominant gene controls the trait. Using a combination of map-based cloning and BSA-seq (Bulked Segregant Analysis -Sequencing), we identified the candidate gene, , responsible for the mutation. Sequence alignment revealed one base substitution in gene , resulting in an amino acid substitution. The deduced amino acid sequence of encodes a HD-DDT (homeodomain-DDT) transcriptional regulatory protein containing a conserved homeobox domain and a DDT domain. Gene expression analysis revealed that the expression level of in the mutant was similar to that in the WT (wild type). Transcriptome analysis indicated that the gene may regulate the development of the epidermis by influencing plant hormone signaling pathways or participating in several transcription factor pathways. The results of this study are fundamental for a better understanding of the function of the HD-DDT transcription factor in the trichome development of cucumber.
茸毛是一种天然的物理屏障，保护植物免受环境压力、自然侵害、紫外线和致病性的影响。茸毛还有助于植物通过降低蒸腾速率来保持适当的含水量。拟南芥单细胞茸毛发育的分子机制已被广泛阐明，但调节多细胞茸毛发育的分子机制尚不清楚。在本研究中，我们从黄瓜 EMS（乙基甲磺酸）诱变群体中鉴定出一个 () 突变体。遗传分析表明，一个不完全显性基因控制着 性状。我们采用图位克隆和 BSA-seq（BSA-测序）相结合的方法，鉴定出候选基因 ，该基因负责 突变。序列比对显示，基因 中存在一个碱基替换，导致氨基酸替换。 基因编码的推定氨基酸序列包含一个保守的同源域和一个 DDT 结构域，是一个 HD-DDT（同源域-DDT）转录调控蛋白。基因表达分析表明， 突变体中 的表达水平与 WT（野生型）相似。转录组分析表明，该 基因可能通过影响植物激素信号通路或参与几个转录因子通路来调节表皮的发育。本研究的结果为更好地理解 HD-DDT 转录因子在黄瓜茸毛发育中的功能提供了基础。
