全基因组条件性小鼠敲除资源
Genome Wide Conditional Mouse Knockout Resources.
作者信息
Kaloff C, Anastassiadis K, Ayadi A, Baldock R, Beig J, Birling M-C, Bradley A, Brown S, Bürger A, Bushell W, Chiani F, Collins F S, Doe B, Eppig J T, Finnel R H, Fletcher C, Flicek P, Fray M, Friedel R H, Gambadoro A, Gates H, Hansen J, Herault Y, Hicks G G, Hörlein A, Hrabé de Angelis M, Iyer V, de Jong P J, Koscielny G, Kühn R, Liu P, Lloyd K C, Lopez R G, Marschall S, Martínez S, McKerlie C, Meehan T, von Melchner H, Moore M, Murray S A, Nagy A, Nutter Lmj, Pavlovic G, Pombero A, Prosser H, Ramirez-Solis R, Ringwald M, Rosen B, Rosenthal N, Rossant J, Ruiz Noppinger P, Ryder E, Skarnes W C, Schick J, Schnütgen F, Schofield P, Seisenberger C, Selloum M, Smedley D, Simpson E M, Stewart A F, Teboul L, Tocchini Valentini G P, Valenzuela D, West A, Wurst W
机构信息
Institute of Developmental Genetics, Helmholtz Zentrum Muenchen, D-85764 Neuherberg, Germany.
Biotechnology Center (BIOTEC) of the Technische Universität Dresden, 01307 Dresden, Germany.
出版信息
Drug Discov Today Dis Models. 2016 Summer;20:3-12. doi: 10.1016/j.ddmod.2017.08.002. Epub 2017 Sep 12.
The International Knockout Mouse Consortium (IKMC) developed high throughput gene trapping and gene targeting pipelines that produced mostly conditional mutations of more than 18,500 genes in C57BL/6N mouse embryonic stem (ES) cells which have been archived and are freely available to the research community as a frozen resource. From this unprecedented resource more than 6,000 mutant mouse strains have been produced by the IKMC and mostly the International Mouse Phenotyping Consortium (IMPC). In addition, a cre-driver resource was established including 250 inducible cre-driver mouse strains in a C57BL/6 background. Complementing the cre-driver resource, a collection of comprising 27 cre-driver rAAVs has also been produced. The resources can be easily accessed at the IKMC/IMPC web portal (www.mousephenotype.org). The IKMC/IMPC resource is a standardized reference library of mouse models with defined genetic backgrounds that enables the analysis of gene-disease associations in mice of different genetic makeup and should therefore have a major impact on biomedical research.
国际基因敲除小鼠联盟（IKMC）开发了高通量基因捕获和基因靶向流程，在C57BL/6N小鼠胚胎干细胞中产生了超过18500个基因的大多数条件性突变，这些细胞已存档，并作为冷冻资源免费提供给研究界。IKMC以及主要是国际小鼠表型分析联盟（IMPC）利用这一前所未有的资源培育出了6000多个突变小鼠品系。此外，还建立了一个cre驱动资源库，其中包括250个C57BL/6背景下的可诱导cre驱动小鼠品系。作为cre驱动资源的补充，还制备了一组包含27个cre驱动rAAV的集合。这些资源可在IKMC/IMPC门户网站（www.mousephenotype.org）上轻松获取。IKMC/IMPC资源是一个具有明确遗传背景的标准化小鼠模型参考库，能够分析不同基因组成小鼠中的基因-疾病关联，因此应该会对生物医学研究产生重大影响。
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