RB51外膜蛋白与S99脂多糖诱导结合后血清的杀菌活性
Bactericidal Activity of Serum by RB51 Outer Membrane Protein's Combined by S99 Lipopolysaccharide Induction.
作者信息
Hajizadeh Sisakht Behnam, Khaledi Mansoor, Afkhami Hamed, Rouhi Saber, Sepehrnia Saeed, Fanaee Vahideh, Karimi Hannaneh, Malekzadegan Yalda, Fathi Javad, Sadati Mahdi S
机构信息
Department of Bacteriology, Faculty of Veterinary, Islamic Azad University, Science & Research Campus, Tehran, Iran.
Department of Microbiology and Immunology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
出版信息
Avicenna J Med Biotechnol. 2024 Jul-Sep;16(3):187-192. doi: 10.18502/ajmb.v16i3.15745.
BACKGROUND
Brucellosis vaccines are designed to induce cellular immunity. An effective brucellosis vaccine could induce both cellular and humoral immunity. Serum Bactericidal Assay (SBA) is an important method for determining vaccine humoral immunity. This study is the first to observe humoral immunity in brucellosis by SBA.
METHODS
Extracted () Lipopolysaccharide (LPS) and Outer Membrane Proteins (OMPs) were injected into rabbits. Group 1 was injected with 25 of LPS, Group 2 was injected with 50 of OMPs, and Group 3 was injected with 1 of combined vaccine, 3 times every 2 weeks. The groups were challenged with 544 in the second injection. Sera were separated 2 weeks after the last injection. SBA was performed, and each well was streak-cultured into a plate of Brucella agar. A colony count was done for each plate.
RESULTS
Results have shown, the third injection of the combined vaccine had the highest titer of , and the efficacy of the vaccine was 87.71%.
CONCLUSION
As a conclusion, the results of this study showed that LPS and OMP's from can provide acceptable immunity.
背景
布鲁氏菌病疫苗旨在诱导细胞免疫。一种有效的布鲁氏菌病疫苗应能同时诱导细胞免疫和体液免疫。血清杀菌试验（SBA）是测定疫苗体液免疫的重要方法。本研究首次通过SBA观察布鲁氏菌病的体液免疫。
方法
提取（）脂多糖（LPS）和外膜蛋白（OMPs）并注射到兔子体内。第1组注射25μg LPS，第2组注射50μg OMPs，第3组注射1μg联合疫苗，每2周注射3次。在第二次注射时用544对各组进行攻击。在最后一次注射后2周分离血清。进行SBA，并将每个孔划线培养到一盘布鲁氏菌琼脂平板上。对每个平板进行菌落计数。
结果
结果显示，第三次注射联合疫苗时滴度最高，为，疫苗效力为87.71%。
结论
总之，本研究结果表明，来自的LPS和OMPs可提供可接受的免疫力。
需注意，原文中存在部分括号缺失内容，翻译时保留了原文的不完整性。
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