Department of Medical Laboratories Techniques, Baqubah Technical Institute, Middle Technical University, Diyala, Iraq.
Community Health Department, Middle Technical University, Technical Institute, Diyala, Iraq.
Mol Biol Rep. 2024 Aug 12;51(1):903. doi: 10.1007/s11033-024-09837-3.
Burns are a problem that affects millions of individuals around the world.
This research aimed to analyze the genetic characteristics and antibiotic resistance of Staphylococcus aureus strains isolated from patients with burns. Identifying the genetic variations of three local strains of Staphylococcus aureus isolated from burns.
Swab samples were collected from eighty sources (burns). Using sterile swabs containing media collected from patients treated at Baqubah Teaching Hospital between July 2022 and the end of September 2022, these samples were then cultured on blood agar and brain heart infusion agar. A total of twenty-four hours were spent incubating the cultured samples in an aerobic environment at 37 °C. During this time, isolated growing colonies showed characteristic growth, color, and hemolysis, while suspicious colonies were cultured for further identification.
Our results indicated the presence of several polymorphisms that were distributed in the investigated samples. However, almost all observed variations were concentrated only in the S2 isolates. The construction of phylogenetic trees confirmed this notion by positioning these S2-based amplicons to distinct categories within Staph. aureus organisms. Furthermore, the phylogenetic tree offered additional tools for the guaranteed identity of the samples that were analyzed. Consequently, the utilization of the PCR-sequencing approach in three DNA samples belonging to these local bacterial isolates has resulted in the confirmation of the identity of this strain. However, particular emphasis should be placed on S2 isolate as it has special variants that differ from its mates, in terms of its metabolic as well as phylogenetic consequences. Therefore, S2 isolates may represent a new strain that requires a whole genome sequencing strategy to validate its identity within Staph. aureus organisms. S.aureus resistance was 100% (Augmentin and Tetracycline), and 90% (Azithromycin and Trimethoprim), while Cefotaxime and Chloramphenicol recorded (75%, and 85%) respectively.
烧伤是一个影响全球数百万人的问题。
本研究旨在分析从烧伤患者中分离出的金黄色葡萄球菌菌株的遗传特征和抗生素耐药性。鉴定从烧伤中分离出的三种本地金黄色葡萄球菌菌株的遗传变异。
从 80 个来源（烧伤）采集拭子样本。使用含有从 2022 年 7 月至 2022 年 9 月底在巴古拜教学医院接受治疗的患者的培养基的无菌拭子采集这些样本，然后将其接种在血琼脂和脑心浸液琼脂上。将培养的样本在 37°C 的有氧环境中孵育 24 小时。在此期间，分离出的生长菌落表现出特征性的生长、颜色和溶血，而可疑的菌落则进行进一步鉴定。
我们的结果表明，在所研究的样本中存在几种分布不均的多态性。然而，几乎所有观察到的变异都仅集中在 S2 分离株中。系统发育树的构建通过将这些基于 S2 的扩增子定位到金黄色葡萄球菌生物体的不同类别中，证实了这一观点。此外，系统发育树还为分析样本的身份提供了额外的工具。因此，在属于这些本地细菌分离株的三个 DNA 样本中使用 PCR-测序方法已证实该菌株的身份。然而，应特别注意 S2 分离株，因为它具有与其伙伴不同的特殊变体，无论是在其代谢还是系统发育方面。因此，S2 分离株可能代表一种新的菌株，需要采用全基因组测序策略来验证其在金黄色葡萄球菌生物体中的身份。金黄色葡萄球菌的耐药率为 100%（氨苄西林和四环素）和 90%（阿奇霉素和甲氧苄啶），而头孢噻肟和氯霉素的耐药率分别为 75%和 85%。
