Zhao Yajing, Zhao Yapeng, Liu Lisheng, Li Guanghao, Wu Yawen, Cui Yanan, Xie Li
Department of Clinical Laboratory, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, China.
Department of Stomatology, Qinghai Red Cross Hospital, Xining, Qinghai, China.
Clin Transl Oncol. 2025 Mar;27(3):1185-1197. doi: 10.1007/s12094-024-03647-6. Epub 2024 Aug 12.
Tumor-derived exosomal miRNAs play crucial roles in cancer diagnosis. Current studies aim to identify exosomal miRNAs associated with colorectal cancer (CRC) that are noninvasive, sensitive, and specific.
Exosomes were extracted from CRC patients and healthy donors via ultracentrifugation, followed by verification via transmission electron microscopy (TEM), qNano, and Western blot analysis. The differential expression levels and clinical characteristics of miR-205-5p were analyzed in CRC via data from The Cancer Genome Atlas (TCGA). Real-time quantitative PCR was used to assess the expression levels of exosomal miRNAs in 157 primary CRC patients, 20 patients with benign diseases, and 135 healthy donors. Predictions regarding target genes were made to guide further exploration of the disease's etiopathogenesis through bioinformatics.
Compared with that in healthy donors, the expression of miR-205-5p in colorectal cancer (CRC) patients was significantly lower, as determined through analysis of the TCGA database. We conducted a prediction and analysis of the functional enrichment of downstream target genes regulated by miR-205-5p. A lower level of exosomal miR-205-5p in the serum of CRC patients than in that of healthy controls (p < 0.0001) and patients with benign disease (p < 0.0001) was observed. Furthermore, the expression levels of exosomal miR-205-5p were significantly lower in early-stage CRC patients than in the comparison groups (p<0.001 and p < 0.0001). Notably, the expression levels of exosomal miR-205-5p significantly increased postoperatively (p = 0.0053).
The present study demonstrated that serum exosomal miR-205-5p may be a diagnostic biomarker for CRC.
肿瘤来源的外泌体微小RNA（miRNA）在癌症诊断中发挥着关键作用。目前的研究旨在鉴定与结直肠癌（CRC）相关的非侵入性、敏感且特异的外泌体miRNA。
通过超速离心从CRC患者和健康供体中提取外泌体，随后通过透射电子显微镜（TEM）、qNano和蛋白质印迹分析进行验证。通过癌症基因组图谱（TCGA）的数据分析CRC中miR-205-5p的差异表达水平和临床特征。采用实时定量PCR评估157例原发性CRC患者、20例良性疾病患者和135例健康供体外泌体miRNA的表达水平。通过生物信息学对靶基因进行预测，以指导对该疾病病因发病机制的进一步探索。
通过对TCGA数据库的分析确定，与健康供体相比，结直肠癌（CRC）患者中miR-205-5p的表达显著降低。我们对miR-205-5p调控的下游靶基因进行了功能富集预测和分析。观察到CRC患者血清中外泌体miR-205-5p水平低于健康对照（p < 0.0001）和良性疾病患者（p < 0.0001）。此外，早期CRC患者中外泌体miR-205-5p的表达水平明显低于对照组（p < 0.001和p < 0.0001）。值得注意的是，外泌体miR-205-5p的表达水平在术后显著升高（p = 0.0053）。
本研究表明血清外泌体miR-
