General Second Disease Area, The First Affiliated Hospital of Henan University of CM, Zhengzhou City, Henan Province, China.
Eur Rev Med Pharmacol Sci. 2020 Jan;24(2):664-672. doi: 10.26355/eurrev_202001_20043.
Emerging evidence has indicated that serum exosomal microRNAs (miRNAs) have promising diagnostic and prognostic value for colorectal cancer (CRC). This study aimed to detect serum exosomal miR-874 expression in CRC patients and assess its potential clinical significance.
Blood samples were collected from 125 CRC patients, 45 cases with benign adenomas (AD) and 70 healthy individuals. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was performed to examine serum exosomal miR-874 levels.
The results showed that serum exosomal miR-874 levels were significantly downregulated in CRC patients compared to AD cases and healthy controls. Receiver operating characteristic (ROC) curve analysis revealed that serum exosomal miR-874 expression could discriminate CRC patients from healthy controls, as well as patients with AD. In addition, low serum exosomal miR-874 expression was associated with positive distant metastasis, positive lymph node metastasis, poor differentiation, and advanced TNM stage. Moreover, serum exosomal miR-874 expression was identified as a statistically significant independent prognostic factor for overall survival of CRC patients.
Collectively, serum exosomal miR-874 expression might serve as a reliable marker for CRC diagnosis and prognosis prediction.
新出现的证据表明，血清外泌体 microRNAs（miRNAs）对结直肠癌（CRC）具有有前途的诊断和预后价值。本研究旨在检测 CRC 患者血清外泌体 miR-874 的表达，并评估其潜在的临床意义。
收集了 125 例 CRC 患者、45 例良性腺瘤（AD）患者和 70 例健康个体的血液样本。采用定量逆转录聚合酶链反应（qRT-PCR）检测血清外泌体 miR-874 水平。
结果显示，CRC 患者血清外泌体 miR-874 水平明显低于 AD 病例和健康对照组。受试者工作特征（ROC）曲线分析表明，血清外泌体 miR-874 表达可区分 CRC 患者与健康对照者，以及 AD 患者。此外，低血清外泌体 miR-874 表达与阳性远处转移、阳性淋巴结转移、低分化和晚期 TNM 分期相关。此外，血清外泌体 miR-874 表达被确定为 CRC 患者总生存期的统计学显著独立预后因素。
综上所述，血清外泌体 miR-874 表达可能是 CRC 诊断和预后预测的可靠标志物。
