Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, 12613, Egypt.
Department of Animal Production, National Research Centre, Dokki, Giza, Egypt.
Trop Anim Health Prod. 2024 Aug 12;56(7):239. doi: 10.1007/s11250-024-04085-w.
Genetic improvement of local rabbit breeds using modern approaches such as marker-assisted selection requires accurate and precise information about marker‒trait associations in animals with different genetic backgrounds. Therefore, this study was designed to estimate the association between two mutations located in the Neuropeptide Y (NPY, g.1778G > C) and Phosphoglycerate Mutase 2 (PGAM2, c.195 C > T) genes in New Zealand White (NZW), Baladi (BR), and V-line rabbits. The first mutation was genotyped using high-resolution melting, and the second mutation was genotyped using the PCR-RFLP method. The results revealed significant associations between the NPY mutation and body weight at 10 (V-line) and 12 weeks of age (NZW, BR, and V-line), body weight gain (BWG) from 10 to 12 weeks of age (BR), BWG from 6 to 12 weeks of age (NZW, BR, and V-line), average daily gain (NZW, BR, and V-line, and BR), growth rate (GR) from 8 to10 weeks (V-line), 10 to 12 weeks (BR), and GR from 6 to 12 weeks of age (BR, and V-line). The PGAM2 mutation was associated with body weight at 10 (V-line) and 12 (NZW, and V-line) weeks of age, with significant positive additive effects at 12 weeks of age in all breeds, and was associated with BWG from 8 to 10 and 10 to 12 in BR, and BWG from 6 to 12 weeks of age (NZW, and BR), and average daily gain (NZW, and BR), and was associated with GR form 8 to 10 weeks (BR), from10 to 12 weeks (BR, and V-line) and from 6 to 12 weeks (BR). The results highlighted the importance of the two mutations in growth development, and the possibility of considering them as candidate genes for late growth in rabbits.
利用现代方法（如标记辅助选择）对地方兔品种进行遗传改良，需要对不同遗传背景下动物的标记与性状之间的关联有准确而精确的信息。因此，本研究旨在估计位于神经肽 Y (NPY，g.1778G > C) 和磷酸甘油酸变位酶 2 (PGAM2，c.195C > T) 基因中的两个突变在新西兰白兔 (NZW)、Baladi (BR) 和 V 线兔中的关联。第一个突变使用高分辨率熔解曲线进行基因分型，第二个突变使用 PCR-RFLP 方法进行基因分型。结果表明，NPY 突变与 10 周龄 (V 线) 和 12 周龄 (NZW、BR 和 V 线) 的体重、10 至 12 周龄的体重增加 (BWG)、6 至 12 周龄的 BWG (NZW、BR 和 V 线)、平均日增重 (NZW、BR 和 V 线，BR)、8 至 10 周龄的生长率 (GR) (V 线)、10 至 12 周龄的生长率 (BR) 以及 6 至 12 周龄的生长率 (BR 和 V 线) 之间存在显著关联。PGAM2 突变与 10 周龄 (V 线) 和 12 周龄 (NZW 和 V 线) 的体重有关，在所有品种的 12 周龄时具有显著的正加性效应，与 BR 中 8 至 10 周龄和 10 至 12 周龄的 BWG 以及 6 至 12 周龄的 BWG 有关，与平均日增重 (NZW 和 BR) 有关，与 8 至 10 周龄的 GR 有关 (BR)、从 10 到 12 周龄 (BR 和 V 线) 和从 6 到 12 周龄 (BR)。结果强调了这两个突变在生长发育中的重要性，并有可能将它们视为兔子后期生长的候选基因。
