EARLY FLOWERING3-1 抑制 Grain number、plant height 和 heading date7，以促进 ABC1 REPRESSOR1 的表达并调节水稻中的氮吸收。

EARLY FLOWERING3-1 represses Grain number, plant height, and heading date7 to promote ABC1 REPRESSOR1 and regulate nitrogen uptake in rice.

机构信息

Rice Research Institute, Shenyang Agricultural University, Shenyang 110866, China.

Agronomy College, Liaodong University, Dandong, 118003, China.

出版信息

Plant Physiol. 2024 Nov 4;196(3):1857-1868. doi: 10.1093/plphys/kiae416.

DOI:10.1093/plphys/kiae416

PMID:39133898

Abstract

The extensive use of nitrogen fertilizer boosts rice (Oryza sativa) production but also harms ecosystems. Therefore, enhancing crop nitrogen use efficiency is crucial. Here, we performed map-based cloning and identified the EARLY FLOWERING3 (ELF3) like protein-encoding gene OsELF3-1, which confers enhanced nitrogen uptake in rice. OsELF3-1 forms a ternary complex (OsEC) with OsELF4s and OsLUX, the putative orthologs of ELF4 and LUX ARRHYTHMO (LUX) in Arabidopsis (Arabidopsis thaliana), respectively. OsEC directly binds to the promoter of Grain number, plant height, and heading date7 (Ghd7) and represses its expression. Ghd7 encodes a transcription factor that has major effects on multiple agronomic traits. Ghd7 is also a transcriptional repressor and directly suppresses the expression of ABC1 REPRESSOR1 (ARE1), a negative regulator of nitrogen use efficiency. Therefore, targeting the OsEC-Ghd7-ARE1 module offers an approach to enhance nitrogen uptake, presenting promising avenues for sustainable agriculture.

摘要

大量使用氮肥可以提高水稻（Oryza sativa）的产量，但也会危害生态系统。因此，提高作物氮素利用效率至关重要。在这里，我们进行了基于图谱的克隆，并鉴定了 EARLY FLOWERING3（ELF3）样蛋白编码基因 OsELF3-1，它可以增强水稻对氮的吸收。OsELF3-1 与 OsELF4s 和 OsLUX 形成一个三元复合物（OsEC），OsELF4s 和 OsLUX 分别是拟南芥（Arabidopsis thaliana）中 ELF4 和 LUX ARRHYTHMO（LUX）的假定同源物。OsEC 直接结合 Grain number, plant height, and heading date7（Ghd7）的启动子并抑制其表达。Ghd7 编码一种转录因子，对多个农艺性状有重要影响。Ghd7 也是一个转录抑制子，直接抑制氮素利用效率负调控因子 ABC1 REPRESSOR1（ARE1）的表达。因此，靶向 OsEC-Ghd7-ARE1 模块提供了一种提高氮吸收的方法，为可持续农业提供了有前景的途径。