Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, School of Life Science and Health Engineering, Jiangnan University, Wuxi 214122, China.
Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, School of Life Science and Health Engineering, Jiangnan University, Wuxi 214122, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Jan 5;324:124950. doi: 10.1016/j.saa.2024.124950. Epub 2024 Aug 8.
Low fluorescence under visible light excitation and catalytic activity limit many applications of graphene quantum dots in optical detection, biosensing, catalysis and biomedical. The paper reports design and synthesis of histidine, serine and folic acid-functionalized and boron and iron-doped graphene quantum dot (Fe/B-GQD-HSF). The Fe/B-GQD-HSF shows excellent fluorescence behavior and peroxidase-like activity. Excitation of 330 nm ultraviolet light produces the strongest blue fluorescence and excitation of 480 nm visible light produces the strongest yellow fluorescence. The specific activity reaches 92.67 U g, which is higher than that of other graphene quantum dots. The Fe/B-GQD-HSF can catalyze oxidation of 3,3',5,5'-tetramethylbenzidine with HO to form blue compound. Based on this, it was used for colorimetric and fluorescence detection of HO. The absorbance at 652 nm linearly increases with the increase of HO concentration between 0.5 and 100 μM with detection limit of 0.43 μM. The fluorescence signal linearly decreases with the increase of HO concentration between 0.05 and 100 μM with detection limit of 0.035 μM. The analytical method has been satisfactorily applied in detection of HO in food. The study also paves one way for design and synthesis of functional graphene quantum dots with ideal fluorescence behavior and catalytic activity.
在可见光激发下的低荧光和催化活性限制了石墨烯量子点在光学检测、生物传感、催化和生物医学中的许多应用。本文报道了组氨酸、丝氨酸和叶酸功能化以及硼和铁掺杂石墨烯量子点（Fe/B-GQD-HSF）的设计与合成。Fe/B-GQD-HSF 表现出优异的荧光行为和过氧化物酶样活性。330nm 紫外光激发产生最强的蓝光荧光，480nm 可见光激发产生最强的黄光荧光。比表面积达到 92.67 U g，高于其他石墨烯量子点。Fe/B-GQD-HSF 可以催化 HO 氧化 3,3',5,5'-四甲基联苯胺生成蓝色化合物。基于此，它被用于 HO 的比色和荧光检测。在 0.5 至 100μM 范围内，652nm 处的吸光度随 HO 浓度的增加呈线性增加，检测限为 0.43μM。在 0.05 至 100μM 范围内，荧光信号随 HO 浓度的增加呈线性降低，检测限为 0.035μM。该分析方法已成功应用于食品中 HO 的检测。该研究也为设计和合成具有理想荧光行为和催化活性的功能化石墨烯量子点铺平了道路。
