Center for Engineering for Medicine and Surgery, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA; Shriners Children's Boston, Boston, MA, USA; Department of Plastic, Reconstructive and Hand Surgery, University Medical Center Utrecht, Utrecht, the Netherlands; Vascularized Composite Allotransplantation Laboratory, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Shriners Children's Boston, Boston, MA, USA; Vascularized Composite Allotransplantation Laboratory, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA; Department of Plastic, Reconstructive et Aesthetic Surgery, Hôpital Paris Saint-Joseph, Paris, France.
Cryobiology. 2024 Sep;116:104950. doi: 10.1016/j.cryobiol.2024.104950. Epub 2024 Aug 24.
Ischemia is a major limiting factor in Vascularized Composite Allotransplantation (VCA) as irreversible muscular injury can occur after as early as 4-6 h of static cold storage (SCS). Organ preservation technologies have led to the development of storage protocols extending rat liver ex vivo preservation up to 4 days. Development of such a protocol for VCAs has the added challenge of inherent ice nucleating factors of the graft, therefore, this study focused on developing a robust protocol for VCA supercooling. Rodent partial hindlimbs underwent subnormothermic machine perfusion (SNMP) with several loading solutions, followed by a storage solution with cryoprotective agents (CPA) developed for VCAs. Storage occurred in suspended animation for 24h and VCAs were recovered using SNMP with modified Steen. This study shows a robust VCA supercooling preservation protocol in a rodent model. Further optimization is expected to allow for its application in a transplantation model, which would be a breakthrough in the field of VCA preservation.
缺血是血管化复合组织同种异体移植（VCA）的主要限制因素，因为在静态冷储存（SCS）后仅 4-6 小时就可能发生不可逆的肌肉损伤。器官保存技术已经导致储存方案的发展，将大鼠肝脏离体保存时间延长至 4 天。开发这样一种 VCAs 的方案具有移植物固有的成冰因子的额外挑战，因此，本研究集中于开发一种强大的 VCA 过冷方案。啮齿动物部分后肢进行亚体温机器灌注（SNMP），并用几种加载溶液，然后用开发用于 VCAs 的含冷冻保护剂（CPA）的储存溶液。储存 24 小时，使用改良的 Steen 进行 SNMP 恢复 VCAs。本研究在啮齿动物模型中展示了一种强大的 VCA 过冷保存方案。进一步的优化有望使其在移植模型中得到应用，这将是 VCA 保存领域的一个突破。
