Department of Diseases and Infection, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo, Japan.
Methods Mol Biol. 2024;2831:301-313. doi: 10.1007/978-1-0716-3969-6_20.
Isolation and culture of dorsal root ganglion (DRG) neurons from adult animals is a useful experimental system for evaluating neural plasticity after axonal injury, as well as the neurological dysfunction resulting from aging and various types of disease. In this chapter, we will introduce a detailed method for the culture of mature rat DRG neurons. About 30-40 ganglia are dissected from a rat and mechanically and enzymatically digested. Subsequently, density gradient centrifugation of the digested tissue using 30% Percoll efficiently eliminates myelin debris and non-neuronal cells, to afford neuronal cells with a high yield and purity.
从成年动物中分离和培养背根神经节（DRG）神经元是评估轴突损伤后神经可塑性以及衰老和各种类型疾病引起的神经功能障碍的有用实验系统。在本章中，我们将介绍一种成熟大鼠 DRG 神经元培养的详细方法。从一只大鼠中解剖出约 30-40 个神经节，然后进行机械和酶消化。随后，使用 30%Percoll 对消化组织进行密度梯度离心，有效地去除了髓鞘碎片和非神经元细胞，从而获得高产率和高纯度的神经元细胞。
