Department of Pharmacology, Laboratory of Neuroanatomy and Neuropsychobiology, Ribeirão Preto Medical School of the University of São Paulo (FMRP-USP), Ribeirão Preto, São Paulo, Brazil.
Behavioural Neuroscience Institute (INeC), São Paulo, Brazil.
Methods Mol Biol. 2024;2831:351-375. doi: 10.1007/978-1-0716-3969-6_24.
Fluorescent and non-fluorescent neural tract tracers enable the investigation of neural pathways in both peripheral and central nervous systems in laboratory animals demonstrating images with high resolution and great anatomic precision. Anterograde and retrograde viral tracers are important cutting-edge tools for neuroanatomical mapping. The optogenetic consists of an advanced alternative for in vivo neural tract tracing procedures, fundamentally considering the possibility to dissect and modulate pathways either exciting or inhibiting neural circuits in laboratory animals. The neurotractography by diffusion tensor imaging in vivo procedures enables the study of neural pathways in humans with reasonable accuracy. Here we describe the procedure of classical anatomic neural tract tracing and modern optogenetic technique performed in anima vili in addition to different diffusion tensor neurotractography performed in anima nobili.
荧光和非荧光神经束示踪剂可用于研究实验室动物的周围和中枢神经系统中的神经通路，显示出具有高分辨率和极好解剖精度的图像。顺行和逆行病毒示踪剂是神经解剖学图谱绘制的重要前沿工具。光遗传学是活体神经束追踪程序的一种先进替代方法，从根本上考虑了在实验室动物中分离和调节兴奋或抑制神经回路的可能性。活体扩散张量成像的神经束追踪程序可在一定程度上准确研究人类的神经通路。在此，我们描述了在实验动物中进行经典解剖神经束追踪和现代光遗传学技术的过程，以及在实验动物中进行不同的扩散张量神经束追踪的过程。
