天然和叠氮修饰的 UDP-鼠李糖和 -阿拉伯呋喃糖的化学合成及其在糖基转移酶糖基化图谱分析中的应用。

Chemical synthesis of natural and azido-modified UDP-rhamnose and -arabinofuranose for glycan array-based characterization of plant glycosyltransferases.

机构信息

Institute of Organic Chemistry, Department of Chemistry, BOKU University, Muthgasse 18, 1190, Vienna, Austria.

Center for Nanomedicine and Theranostics, Department of Chemistry, Technical University of Denmark, Kemitorvet, Building 207, 2800 Kongens Lyngby, Denmark.

出版信息

Chem Commun (Camb). 2024 Aug 27;60(70):9368-9371. doi: 10.1039/d4cc02095b.

DOI:10.1039/d4cc02095b

PMID:39135501

Abstract

Chemical syntheses of UDP-rhamnose and UDP-arabinofuranose and respective azido-modified analogues are reported. The prepared substrates are useful for the glycan array-based analysis of glycosyltransferases, as exemplified with the plant cell wall-biosynthetic enzymes XAT3, RRT4 and RRT5.

摘要

报道了 UDP-鼠李糖和 UDP-阿拉伯呋喃糖及其相应的叠氮修饰类似物的化学合成。所制备的底物可用于糖基转移酶的基于聚糖阵列的分析，如植物细胞壁生物合成酶 XAT3、RRT4 和 RRT5 的实例所示。