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通过重组自交系的混合分离群体分析法，在春小麦PI672538中鉴定并定位了三个新的抗赤霉病QTL。

Three novel QTLs for FHB resistance identified and mapped in spring wheat PI672538 by bulked segregant analysis of the recombinant inbred line.

作者信息

Huang Qianglan, Li Xin, Li Qing, Zhong Shengfu, Li Xiuying, Yang Jiezhi, Tan Feiquan, Ren Tianheng, Li Zhi, Suizhuang Yang

机构信息

Wheat Research Institute, School of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, China.

Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, China.

出版信息

Front Plant Sci. 2024 Jul 29;15:1409095. doi: 10.3389/fpls.2024.1409095. eCollection 2024.

DOI:10.3389/fpls.2024.1409095
PMID:39135653
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11317384/
Abstract

INTRODUCTION

head blight (FHB) has a large influence on both the yield and quality of wheat grain worldwide. Host resistance is the most effective method for controlling FHB, but unfortunately, very few genetic resources on FHB resistance are available; therefore, identifying novel resistance genes or quantitative trait loci (QTLs) is valuable.

METHODS

Here, a recombinant inbred line (RIL) population containing 451 lines derived from the cross L661/PI672538 was sown in four different environments (2019CZ, 2019CZ, 2021QL and 2021WJ).

RESULTS

Five QTLs, consisting of two previously reported QTLs ( and ) and three new QTLs (, and ), were identified. Further investigation revealed that , and could be detected in all four environments, and and FhbL693e were detected only in 2019CZb and 2021WJ, respectively. Among the QTLs, the greatest contribution (10.5%) to the phenotypic variation effect (PVE) was in 2021WJ, while the smallest (1.2%) was and in 2019CZ. The selection of for , for and for decreased the number of damaged spikelets by 2.1, and a new line resistant to FHB named H140-2 was developed by marker-assisted selection (MAS).

DISCUSSION

These results could help to further improve FHB resistance in the future.

摘要

引言

赤霉病（FHB）对全球小麦籽粒的产量和品质都有很大影响。寄主抗性是控制赤霉病最有效的方法，但遗憾的是，可用的抗赤霉病遗传资源非常少；因此，鉴定新的抗性基因或数量性状位点（QTL）很有价值。

方法

在此，将一个由451个株系组成的重组自交系（RIL）群体播种于四个不同环境（2019CZ、2019CZ、2021QL和2021WJ），该群体源自L661/PI672538杂交组合。

结果

鉴定出5个QTL，包括2个先前报道的QTL（和）以及3个新的QTL（、和）。进一步研究发现，、和在所有四个环境中均能检测到，而和FhbL693e分别仅在2019CZb和2021WJ中检测到。在这些QTL中，对表型变异效应（PVE）贡献最大的是2021WJ中的（10.5%），而最小的是2019CZ中的和（1.2%）。选择用于的、用于的和用于的可使受损小穗数减少2.1，并通过标记辅助选择（MAS）培育出一个抗赤霉病的新品系H140 - 2。

讨论

这些结果有助于未来进一步提高对赤霉病的抗性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4560/11317384/431244ac3e93/fpls-15-1409095-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4560/11317384/d581e6e14092/fpls-15-1409095-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4560/11317384/6628c217e1b2/fpls-15-1409095-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4560/11317384/bb584a7a94dc/fpls-15-1409095-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4560/11317384/6257d948ab2b/fpls-15-1409095-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4560/11317384/037a317e735a/fpls-15-1409095-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4560/11317384/431244ac3e93/fpls-15-1409095-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4560/11317384/d581e6e14092/fpls-15-1409095-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4560/11317384/6628c217e1b2/fpls-15-1409095-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4560/11317384/bb584a7a94dc/fpls-15-1409095-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4560/11317384/6257d948ab2b/fpls-15-1409095-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4560/11317384/037a317e735a/fpls-15-1409095-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4560/11317384/431244ac3e93/fpls-15-1409095-g006.jpg

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