Kim Hyungsuk, Lee Kyunghoon, Seo Soo Hyun, Hong Yun Ji, Hwang Sang Mee, Park Jeong Su, Park Kyoung Un, Song Junghan
Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, South Korea.
Department of Laboratory Medicine, Seoul National University Hospital, Seoul, South Korea.
Transfus Med Hemother. 2024 Jan 4;51(4):274-285. doi: 10.1159/000535625. eCollection 2024 Aug.
Di(2-ethylhexyl) phthalate (DEHP) is a plasticizer commonly used in blood bags. Despite its protective effects on red blood cell (RBC) storage, concerns about its reproductive toxicity exist. This study investigated the in vitro quality of RBC concentrates stored in bags using di(isononyl) cyclohexane-1,2-dicarboxylate (DINCH) as an alternative plasticizer.
Using a pool-and-split study design, we produced 20 matched homogenous quintets of RBC concentrates in two DINCH bags and three DEHP bags with citrate phosphate dextrose adenine (CPDA-1) anticoagulant. RBC storage quality was assessed weekly for 35 days.
On day 35, the median hemolysis levels in the DINCH bags (0.297-0.342%) were marginally higher ( < 0.05) than the DEHP bags (0.204-0.240%). All DINCH bags showed <0.8% hemolysis. RBCs in the DINCH bags showed increased mean corpuscular volume and decreased eosin 5' maleimide binding than in the DEHP bags. Higher pO and lower pCO levels in the DINCH bags indicated better gas permeability than in DEHP bags. Other metabolic parameters were comparable in both bags. Compared to DEHP, DINCH exhibited considerably lower levels of plasticizer leaching into blood bags.
The quality of RBC concentrates stored for 35 days in DINCH-plasticized blood bags with CDPA-1 is generally comparable to those in DEHP bags. Hence, DINCH can be a viable alternative to DEHP in blood bags for nonleukoreduced RBC storage even without the use of next-generation additive solutions to improve RBC preservation quality.
邻苯二甲酸二（2-乙基己基）酯（DEHP）是一种常用于血袋的增塑剂。尽管它对红细胞（RBC）储存有保护作用，但人们对其生殖毒性仍存在担忧。本研究调查了使用环己烷-1,2-二羧酸二异壬酯（DINCH）作为替代增塑剂的血袋中储存的红细胞浓缩物的体外质量。
采用混合分割研究设计，我们用含枸橼酸盐磷酸盐葡萄糖腺嘌呤（CPDA-1）抗凝剂的两个DINCH血袋和三个DEHP血袋制备了20组匹配的同质五联红细胞浓缩物。对红细胞储存质量进行了35天的每周评估。
在第35天，DINCH血袋中的溶血水平中位数（0.297 - 0.342%）略高于DEHP血袋（0.204 - 0.240%）（<0.05）。所有DINCH血袋的溶血率均<0.8%。与DEHP血袋相比，DINCH血袋中的红细胞平均体积增加，嗜酸性5'马来酰亚胺结合减少。DINCH血袋中较高的pO和较低的pCO水平表明其透气性优于DEHP血袋。两个血袋中的其他代谢参数相当。与DEHP相比，DINCH向血袋中渗出的增塑剂水平要低得多。
在含CDPA-1的DINCH增塑血袋中储存35天的红细胞浓缩物质量通常与DEHP血袋中的相当。因此，即使不使用下一代添加剂溶液来提高红细胞保存质量，DINCH在用于储存非白细胞滤除红细胞的血袋中也可以成为DEHP的可行替代品。
