Srivastava R, Khan A A, Srivastava B S
Gene. 1985;40(2-3):267-72. doi: 10.1016/0378-1119(85)90049-6.
Antibodies have been used as probe to detect cloned genes coding for toxin and surface antigens of Vibrio cholerae El Tor strain KB207. EcoRI-digested chromosomal DNA of KB207 was cloned in plasmid pBR325 and transformed in Escherichia coli HB101(lambda cI857). Transformants were grown at 32 degrees C on plates containing antibodies. Lysogen was induced at 42 degrees C to release expressed antigens. Antigen-antibody reaction produced a halo around positive clones.
抗体已被用作探针，以检测编码霍乱弧菌El Tor菌株KB207毒素和表面抗原的克隆基因。KB207经EcoRI酶切的染色体DNA被克隆到质粒pBR325中，并转化到大肠杆菌HB101（λcI857）中。转化子在含有抗体的平板上于32℃培养。在42℃诱导溶原菌以释放表达的抗原。抗原-抗体反应在阳性克隆周围产生晕圈。
