Silva Glenda Nicioli da, Pereira Isadora Oliveira Ansaloni, Lima Ana Paula Braga, Almeida Tamires Cunha, Sávio André Luiz Ventura, Costa Renato Prado, Leite Kátia Ramos Moreira, Salvadori Daisy Maria Fávero
Universidade Federal de Ouro Preto, Escola de Farmácia, Departamento de Análises Clínicas, Ouro Preto, MG, Brazil.
Instituto Butantan, Laboratório de Dor e Sinalização, São Paulo, SP, Brazil.
Genet Mol Biol. 2024 Aug 9;47(3):e20230265. doi: 10.1590/1678-4685-GMB-2023-0265. eCollection 2024.
Bladder cancer is the tenth most frequently diagnosed cancer globally. Classification of high- or low-grade tumors is based on cytological differentiation and is an important prognostic factor. LncRNAs regulate gene expression and play critical roles in the occurrence and development of cancer, however, there are few reports on their diagnostic value and co-expression levels with genes, which may be useful as specific biomarkers for prognosis and therapy in bladder cancer. Thus, we performed a marker lesion study to investigate whether gene/lncRNA expression in urothelial carcinoma tissues may be useful in differentiating low-grade and high-grade tumors. RT-qPCR was used to evaluate the expression of the JHDM1D gene and the lncRNAs CTD-2132N18.2, SBF2-AS1, RP11-977B10.2, CTD-2510F5.4, and RP11-363E7.4 in 20 histologically diagnosed high-grade and 10 low-grade tumors. A protein-to-protein interaction network between genes associated with JHDM1D gene was constructed using STRING website. The results showed a moderate (positive) correlation between CTD-2510F5.4 and CTD2132N18.2. ROC curve analyses showed that combined JHDM1D and RP11-363E7.4 predicted tumor grade with an AUC of 0.826, showing excellent accuracy. In conclusion, the results indicated that the combined expression of JHDM1D and RP11-363E7.4 may be a prognostic biomarker and a promising target for urothelial tumor therapy.
膀胱癌是全球第十大最常被诊断出的癌症。高分级或低分级肿瘤的分类基于细胞分化，是一个重要的预后因素。长链非编码RNA（lncRNAs）调节基因表达，并在癌症的发生和发展中起关键作用。然而，关于它们的诊断价值以及与基因的共表达水平的报道很少，而这些可能作为膀胱癌预后和治疗的特异性生物标志物。因此，我们进行了一项标记病变研究，以调查尿路上皮癌组织中的基因/lncRNA表达是否有助于区分低分级和高分级肿瘤。采用逆转录定量聚合酶链反应（RT-qPCR）评估20例组织学诊断为高分级肿瘤和10例低分级肿瘤中JHDM1D基因以及lncRNAs CTD-2132N18.2、SBF2-AS1、RP11-977B10.2、CTD-2510F5.4和RP11-363E7.4的表达。使用STRING网站构建了与JHDM1D基因相关的基因之间的蛋白质-蛋白质相互作用网络。结果显示CTD-2510F5.4和CTD2132N18.2之间存在中度（正）相关。ROC曲线分析表明，联合JHDM1D和RP11-363E7.4预测肿瘤分级的曲线下面积（AUC）为0.826，显示出极佳的准确性。总之，结果表明JHDM1D和RP11-363E7.4的联合表达可能是一种预后生物标志物，也是尿路上皮肿瘤治疗的一个有前景的靶点。
