Schofield G M, Locci R
J Appl Bacteriol. 1985 Dec;59(6):519-27. doi: 10.1111/j.1365-2672.1985.tb03355.x.
Simultaneous experiments were performed with sterilized and non-sterile water and an artificial hard water. After seeding with an environmental isolate of Legionella pneumophila numbers in the sterile and hard water decreased rapidly and colonization of various tap washer fittings failed to take place. Adhesion and growth of an environmental isolate of L. pneumophila to washers in non-sterile tap water was followed over a 4-month period with fluorescein-labelled antibody and by scanning electron microscopy. After adherence the individual cells appeared to divide to form chains which spread over the surfaces. Organisms other than legionellas were also present and a complex colonization matt was formed which was embedded in a protective coat of slime and debris. The numbers of L. pneumophila recovered from the water were highest between 4 and 7 weeks but they could still be cultivated after 4 months.
同时使用无菌水、非无菌水和人工硬水进行了实验。接种嗜肺军团菌的环境分离株后，无菌水和硬水中的菌数迅速下降，各种水龙头垫圈配件均未出现定殖现象。在4个月的时间里，用荧光素标记抗体并通过扫描电子显微镜观察了嗜肺军团菌环境分离株在非无菌自来水中对垫圈的粘附和生长情况。粘附后，单个细胞似乎分裂形成链，这些链蔓延在表面。除军团菌外还存在其他微生物，形成了一个复杂的定殖垫，该定殖垫嵌入粘液和碎屑的保护涂层中。从水中回收的嗜肺军团菌数量在4至7周时最高，但4个月后仍可培养。
