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MYB52 负调控拟南芥 pavement 细胞形态发生过程中 ADF9 介导的肌动蛋白丝束。

MYB52 negatively regulates ADF9-meditated actin filament bundling in Arabidopsis pavement cell morphogenesis.

机构信息

College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, China.

College of Biological Science, China Agricultural University, Beijing, 100091, China.

出版信息

J Integr Plant Biol. 2024 Nov;66(11):2379-2394. doi: 10.1111/jipb.13762. Epub 2024 Aug 13.

DOI:10.1111/jipb.13762
PMID:39136601
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11583842/
Abstract

It has been proposed that cortical fine actin filaments are needed for the morphogenesis of pavement cells (PCs). However, the precise role and regulation mechanisms of actin filaments in PC morphogenesis are not well understood. Here, we found that Arabidopsis thaliana ACTIN DEPOLYMERIZING FACTOR9 (ADF9) is required for the morphogenesis of PC, which is negatively regulated by the R2R3 MYELOBLASTOSIS (MYB) transcription factor MYB52. In adf9 mutants, the lobe number of cotyledon PCs was significantly reduced, while the average lobe length did not differ significantly compared to that of wild type (Col-0), except for the variations in cell area and circularity, whereas the PC shapes in ADF9 overexpression seedlings showed different results. ADF9 decorated actin filaments, and colocalized with plasma membrane. The extent of filament bundling and actin filament bundling activity in adf9 mutant decreased. In addition, MYB52 directly targeted the promoter of ADF9 and negatively regulated its expression. The myb52-2 mutant showed increased lobe number and cell area, reduced cell circularity of PCs, and the PC phenotypes were suppressed when ADF9 was knocked out. Taken together, our data demonstrate that actin filaments play an important role in the morphogenesis of PC and reveal a transcriptional mechanism underlying MYB52 regulation of ADF9-mediated actin filament bundling in PC morphogenesis.

摘要

已经有人提出，皮质细肌动蛋白丝对于 pavement cells (PCs) 的形态发生是必需的。然而，肌动蛋白丝在 PCs 形态发生中的精确作用和调节机制还不是很清楚。在这里，我们发现拟南芥 ACTIN DEPOLYMERIZING FACTOR9 (ADF9) 对于 PCs 的形态发生是必需的，它受到 R2R3 MYELOBLASTOSIS (MYB) 转录因子 MYB52 的负调控。在 adf9 突变体中，子叶 PCs 的叶瓣数量明显减少，而与野生型（Col-0）相比，平均叶瓣长度没有显著差异，除了细胞面积和圆形度的变化外，而 ADF9 过表达幼苗中的 PCs 形状则表现出不同的结果。ADF9 修饰肌动蛋白丝，并与质膜共定位。adf9 突变体中细丝束的程度和肌动蛋白丝束的活性降低。此外，MYB52 直接靶向 ADF9 的启动子并负调控其表达。myb52-2 突变体表现出叶瓣数量增加和细胞面积增大、PCs 细胞圆形度降低的表型，当 ADF9 被敲除时，PC 表型被抑制。总之，我们的数据表明肌动蛋白丝在 PCs 的形态发生中起着重要作用，并揭示了 MYB52 调节 ADF9 介导的肌动蛋白丝束在 PCs 形态发生中的转录机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69fc/11583842/2256381d50f3/JIPB-66-2379-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69fc/11583842/9490dde9e101/JIPB-66-2379-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69fc/11583842/4cd2f7f2c40a/JIPB-66-2379-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69fc/11583842/8734b62a9ba1/JIPB-66-2379-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69fc/11583842/a6f480fbde0e/JIPB-66-2379-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69fc/11583842/e651dcb38187/JIPB-66-2379-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69fc/11583842/e0e250ad126a/JIPB-66-2379-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69fc/11583842/a02b22239ffc/JIPB-66-2379-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69fc/11583842/e4408e9e8132/JIPB-66-2379-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69fc/11583842/2256381d50f3/JIPB-66-2379-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69fc/11583842/9490dde9e101/JIPB-66-2379-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69fc/11583842/4cd2f7f2c40a/JIPB-66-2379-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69fc/11583842/8734b62a9ba1/JIPB-66-2379-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69fc/11583842/a6f480fbde0e/JIPB-66-2379-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69fc/11583842/e651dcb38187/JIPB-66-2379-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69fc/11583842/e0e250ad126a/JIPB-66-2379-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69fc/11583842/a02b22239ffc/JIPB-66-2379-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69fc/11583842/e4408e9e8132/JIPB-66-2379-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69fc/11583842/2256381d50f3/JIPB-66-2379-g004.jpg

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