Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si, Jeollabuk-do 56212, Republic of Korea.
BioMedical Sciences Graduate Program (BMSGP), Chonnam National University, Hwasun 58128, Republic of Korea.
Int J Syst Evol Microbiol. 2024 Aug;74(8). doi: 10.1099/ijsem.0.006489.
A novel, Gram-positive, facultatively anaerobic, and non-motile bacterial strain, designated B2T-5, was isolated from jeotgal, a traditional Korean fermented seafood. Colonies grown on gifu anaerobic medium agar plates were cream-coloured, irregular, and umbonate with curled margins. Optimal growth of strain B2T-5 occurred at 20 °C, pH 8.0, and in the presence of 1% (w/v) NaCl. Strain B2T-5 was negative for oxidase and catalase activity. Hippurate was not hydrolysed and acetoin was not produced. The major cellular fatty acids were C 9 and C. The cell-wall peptidoglycan was of the A4 type containing l-Lys-d-Asp. The predominant respiratory quinone was menaquinone 7. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylcholine. According to the phylogenetic analysis based on 16S rRNA gene sequences, strain B2T-5 was most closely related to DSM 21459, showing 98.2% sequence similarity. Genome sequencing of strain B2T-5 revealed a genome size of 2.0 Mbp and a G+C content of 33.8 mol%. The average nucleotide identities of strain B2T-5 with DSM 21459, SS1994, and D7T301 were 75.0, 74.7, and 75.1%, respectively. Based on the phenotypic, chemotaxonomic, and genotypic data, strain B2T-5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is B2T-5 (=KCTC 21223=JCM 35937).
一株新型革兰氏阳性、兼性厌氧、非运动性的细菌菌株 B2T-5 从韩国传统发酵海鲜珍露中分离得到。在吉富厌氧培养基琼脂平板上生长的菌落呈奶油色、不规则、有脐状凸起且边缘卷曲。菌株 B2T-5 的最佳生长条件为 20°C、pH8.0 和 1%（w/v）NaCl。菌株 B2T-5 氧化酶和过氧化氢酶活性均为阴性。它不能水解马尿酸，也不能产生乙酰醇。主要的细胞脂肪酸为 C9 和 C16:0。细胞壁肽聚糖属于 A4 型，含有 l-Lys-d-Asp。主要的呼吸醌是menaquinone 7。主要的极性脂类为双磷脂酰甘油、磷脂酰甘油和磷脂酰胆碱。根据 16S rRNA 基因序列的系统发育分析，菌株 B2T-5 与 DSM 21459 最为密切相关，序列相似度为 98.2%。B2T-5 菌株的基因组测序揭示了其基因组大小为 2.0 Mbp，GC 含量为 33.8 mol%。B2T-5 菌株与 DSM 21459、SS1994 和 D7T301 的平均核苷酸同一性分别为 75.0%、74.7%和 75.1%。基于表型、化学分类和基因型数据，菌株 B2T-5 代表了属的一个新型种，建议将其命名为 sp. nov.。其模式菌株为 B2T-5（=KCTC 21223=JCM 35937）。
