School of Biological Sciences & Research Center for Biomolecules and Biosystems Chung-Ang University, Seoul 156-756, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Sep;63(Pt 9):3430-3436. doi: 10.1099/ijs.0.049197-0. Epub 2013 Mar 29.
A Gram-staining-positive, aerobic, non-motile bacterium, designated strain SJ5-8(T), was isolated from seau-jeot (shrimp jeotgal), a traditional fermented seafood in South Korea. Cells were non-spore-forming rods showing catalase- and oxidase-positive reactions. Growth of strain SJ5-8(T) was observed at 10-37 °C (optimum, 30 °C), at pH 6.0-9.0 (optimum, pH 7.5-8.5) and in the presence of 0-14% (w/v) NaCl (optimum, 5%). Phylogenetic inference based on 16S rRNA gene sequences showed that the strain formed a tight phyletic lineage with members of the genus Brevibacterium. Strain SJ5-8(T) was most closely related to Brevibacterium yomogidense MN-6-a(T), Brevibacterium daeguense 2C6-41(T) and Brevibacterium salitolerans TRM 415(T) with similarities of 98.9, 97.5 and 97.4%, respectively. The DNA-DNA relatedness values between strain SJ5-8(T) and the type strains of B. yomogidense, B. daeguense and B. salitolerans were 51.7 ± 1.9%, 22.2 ± 4.0% and 52.4 ± 3.8%, respectively. Chemotaxonomic data (major sole isoprenoid quinone, MK-8(H₂); major diagnostic diamino acid, meso-diaminopimelic acid; major polyamines, putrescine and cadaverine; major cellular fatty acids, anteiso-C(15:0), iso-C(15:0) and anteiso-C(17:0); major polar lipids, phosphatidylglycerol and diphosphatidylglycerol; DNA G+C content, 69.3 mol%) also supported the affiliation of strain SJ5-8(T) to the genus Brevibacterium. Therefore, strain SJ5-8(T) represents a novel species of the genus Brevibacterium, for which the name Brevibacterium jeotgali sp. nov. is proposed. The type strain is SJ5-8(T) ( =KACC 16911(T) =JCM 18571(T)).
一株革兰氏阳性、需氧、非运动的细菌，被命名为 SJ5-8(T) 菌株，从韩国传统发酵海鲜虾酱中分离得到。细胞呈无孢子的杆状，呈过氧化氢酶和氧化酶阳性反应。SJ5-8(T) 菌株的生长温度为 10-37°C（最佳温度为 30°C），pH 值为 6.0-9.0（最佳 pH 值为 7.5-8.5），并能在 0-14%（w/v）NaCl（最佳为 5%）中生长。基于 16S rRNA 基因序列的系统发育推断表明，该菌株与短杆菌属的成员形成了一个紧密的系统发育谱系。SJ5-8(T) 菌株与 Brevibacterium yomogidense MN-6-a(T)、Brevibacterium daeguense 2C6-41(T) 和 Brevibacterium salitolerans TRM 415(T) 的相似性最高，分别为 98.9%、97.5%和 97.4%。SJ5-8(T) 菌株与 Brevibacterium yomogidense、Brevibacterium daeguense 和 Brevibacterium salitolerans 型菌株的 DNA-DNA 相关值分别为 51.7±1.9%、22.2±4.0%和 52.4±3.8%。化学分类数据（主要的异戊烯醌，MK-8(H₂)；主要的二氨基庚二酸，meso-二氨基庚二酸；主要的多胺，腐胺和尸胺；主要的细胞脂肪酸，anteiso-C(15:0)、iso-C(15:0)和 anteiso-C(17:0)；主要的极性脂，磷脂酰甘油和双磷脂酰甘油；DNA G+C 含量，69.3 mol%）也支持 SJ5-8(T) 菌株属于短杆菌属。因此，SJ5-8(T) 菌株代表了短杆菌属的一个新种，建议将其命名为 Brevibacterium jeotgali sp. nov. 。其模式菌株为 SJ5-8(T)（=KACC 16911(T)=JCM 18571(T)）。
