CSIR- Institute of Himalayan Bioresource Technology, Palampur, 176061, HP, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
CSIR- Institute of Himalayan Bioresource Technology, Palampur, 176061, HP, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
Plant Physiol Biochem. 2024 Oct;215:109031. doi: 10.1016/j.plaphy.2024.109031. Epub 2024 Aug 8.
Drought is a major abiotic stress that occurs frequently due to climate change, severely hampers agricultural production, and threatens food security. In this study, the effect of drought-tolerant PGPRs, i.e., PGPR-FS2 and PGPR-VHH4, was assessed on wheat by withholding water. The results indicate that drought-stressed wheat seedlings treated with PGPRs-FS2 and PGPR-VHH4 had a significantly higher shoot and root length, number of roots, higher chlorophyll, and antioxidant enzymatic activities of guaiacol peroxidase (GPX) compared to without PGPR treatment. The expression study of wheat genes related to tryptophan auxin-responsive (TaTAR), drought-responsive (TaWRKY10, TaWRKY51, TaDREB3, and TaDREB4) and auxin-regulated gene organ size (TaARGOS-A, TaARGOS-B, and TaARGOS-D) exhibited significantly higher expression in the PGPR-FS2 and PGPR-VHH4 treated wheat under drought as compared to without PGPR treatment. The results of this study illustrate that PGPR-FS2 and PGPR-VHH4 mitigate the drought stress in wheat and pave the way for imparting drought in wheat under water deficit conditions. Among the two PGPRs, PGPR-VHH4 more efficiently altered the root architecture to withstand drought stress.
干旱是一种由于气候变化而频繁发生的主要非生物胁迫，严重阻碍农业生产，威胁粮食安全。在本研究中，通过断水评估了耐旱 PGPR，即 PGPR-FS2 和 PGPR-VHH4，对小麦的影响。结果表明，与未用 PGPR 处理的干旱胁迫小麦幼苗相比，用 PGPR-FS2 和 PGPR-VHH4 处理的小麦幼苗的地上部和根长、根数量、更高的叶绿素和抗氧化酶苯丙氨酸解氨酶（GPX）活性显著提高。与未用 PGPR 处理的干旱胁迫小麦相比，与色氨酸生长素响应（TaTAR）、干旱响应（TaWRKY10、TaWRKY51、TaDREB3 和 TaDREB4）和生长素调节基因器官大小（TaARGOS-A、TaARGOS-B 和 TaARGOS-D）相关的小麦基因的表达研究表明，用 PGPR-FS2 和 PGPR-VHH4 处理的小麦中这些基因的表达显著升高。本研究结果表明，PGPR-FS2 和 PGPR-VHH4 减轻了小麦的干旱胁迫，为在缺水条件下赋予小麦耐旱性铺平了道路。在这两种 PGPR 中，PGPR-VHH4 更有效地改变了根系结构以抵御干旱胁迫。
