Key Laboratory of Three Gorges Regional Plant Genetics and Germplasm Enhancement (CTGU)/Biotechnology Research Center, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, China.
J Agric Food Chem. 2024 Aug 28;72(34):19232-19245. doi: 10.1021/acs.jafc.4c03411. Epub 2024 Aug 13.
Plant cell culture technology helps to obtain natural plant-derived metabolites. The callus of sorghum, a prominent cereal crop, possesses various metabolites with potential health benefits. However, the epigenetic mechanism regulating metabolic biosynthetic capabilities in sorghum remains unknown. Therefore, we conducted N6-methyladenine (6mA) methylome analysis using transcriptome profiling and metabolome analysis to investigate the role of 6mA alterations in two calluses having different biosynthetic capacities, which were derived from immature sorghum embryos. Our findings indicate that the 6mA upregulation within gene bodies is crucial in transcriptional activity potentially mediated by the DNA demethylase SbALKBH1. Furthermore, 6mA was significantly enriched in genes involved in the biosynthesis of flavonoids and isoflavonoids. This could serve as a novel source of bioactive compounds for human health. Thus, 6mA could play an essential role in flavonoid biosynthesis in the sorghum callus.
植物细胞培养技术有助于获取天然植物衍生的代谢产物。高粱是一种重要的谷物作物，其愈伤组织具有多种具有潜在健康益处的代谢产物。然而，调节高粱代谢生物合成能力的表观遗传机制尚不清楚。因此，我们使用转录组谱分析和代谢组分析进行了 N6-甲基腺嘌呤（6mA）甲基组分析，以研究在两个具有不同生物合成能力的愈伤组织中，6mA 改变在其中的作用，这两个愈伤组织是从不成熟的高粱胚胎中获得的。我们的研究结果表明，基因体内的 6mA 上调对于转录活性至关重要，这可能是由 DNA 去甲基酶 SbALKBH1 介导的。此外，6mA 显著富集在参与类黄酮和异黄酮生物合成的基因中。这可以作为人类健康的生物活性化合物的新来源。因此，6mA 可能在高粱愈伤组织的类黄酮生物合成中发挥重要作用。
