College of Health Science and Engineering, Hubei University, Wuhan, Hubei Province, P. R. China.
College of Food Science and Technology/National R&D Branch Center for Conventional Freshwater Fish Processing (Wuhan), Huazhong Agricultural University, Wuhan, Hubei Province, P. R. China.
J Food Sci. 2024 Sep;89(9):5473-5487. doi: 10.1111/1750-3841.17090. Epub 2024 Aug 13.
Warmed-over flavor (WOF) is an off-flavor in surimi gels. Yeast extract (YE) could improve the aroma properties of food. However, the effect of YE on the WOF in surimi gels and its mechanism was still unclear. In this study, aroma profiles, the composition of aroma compounds and aroma precursors, concentrations of WOF compounds, and thiobarbituric acid reactive substances (TBARS) of surimi gels with different amounts of YE were investigated by molecular sensory science and chromatographic techniques. Moreover, the effect of pyrazines and esters introduced by YE on WOF was also tested by sensory analysis. The addition of no less than 1% YE to surimi gels significantly weakened WOF. However, YE did not decrease the concentrations of WOF compounds and did not change the fatty acid composition and TBARS in surimi gels. Conversely, the addition of YE significantly increased the contents of free amino acids, N-containing compounds, and esters in surimi gels. The contents of total free amino acids, 2,6-dimethylpyrazine, and ethyl acetate in surimi gels with 2.5% YE were 1.5, 21, and 2.1 times higher than those in the control, respectively. Additionally, the sensory results of the spiked aroma models containing WOF compounds, 2,6-dimethylpyrazine, and esters showed that more than 9.4 µg/kg of 2,6-dimethylpyrazine with a baked-potato note and more than 6.1 µg/kg of ethyl acetate and 11.2 µg/kg of butyl acetate with a fruity note could significantly mask WOF. In conclusion, WOF in surimi gels could be masked by YE due to the high concentrations of pyrazines and esters. Practical Application: Yeast extracts could decrease the warmed-over flavor (WOF) due to the high concentrations of pyrazines (baked-potato note) and esters (fruity note). This finding extends the application of yeast extracts in the food industry. On the other hand, this study presents a reasonable solution for the reduction of WOF in surimi products.
回锅味是鱼糜凝胶中的一种异味。酵母抽提物(YE)可以改善食品的香气特性。然而,YE 对鱼糜凝胶中回锅味的影响及其机制尚不清楚。在本研究中,通过分子感官科学和色谱技术研究了不同添加量 YE 的鱼糜凝胶的香气特征、香气化合物和香气前体组成、回锅味化合物浓度和硫代巴比妥酸反应物(TBARS)。此外,还通过感官分析测试了 YE 引入的吡嗪和酯对回锅味的影响。向鱼糜凝胶中添加不少于 1%的 YE 可显著减弱回锅味。然而,YE 并没有降低回锅味化合物的浓度,也没有改变鱼糜凝胶中的脂肪酸组成和 TBARS。相反,YE 的添加显著增加了鱼糜凝胶中游离氨基酸、含氮化合物和酯的含量。添加 2.5% YE 的鱼糜凝胶中总游离氨基酸、2,6-二甲基吡嗪和乙酸乙酯的含量分别比对照提高了 1.5、21 和 2.1 倍。此外,含有回锅味化合物、2,6-二甲基吡嗪和酯的加香模型的感官结果表明,超过 9.4µg/kg 具有烤土豆味的 2,6-二甲基吡嗪、超过 6.1µg/kg 具有水果味的乙酸乙酯和 11.2µg/kg 具有水果味的丁酸乙酯可以显著掩盖回锅味。综上所述,由于吡嗪和酯的高浓度,YE 可以掩盖鱼糜凝胶中的回锅味。实际应用:酵母抽提物可以通过高浓度的吡嗪(烤土豆味)和酯(水果味)来降低回锅味。这一发现扩展了酵母抽提物在食品工业中的应用。另一方面,本研究为减少鱼糜制品中的回锅味提供了合理的解决方案。