Exploring the influence of deposit mineral composition on biofilm communities in oil and gas systems.

INTRODUCTION

Inside oil and gas pipelines, native microbial communities and different solid compounds typically coexist and form mixed deposits. However, interactions between these deposits (primarily consisting of mineral phases) and microorganisms in oil and gas systems remain poorly understood. Here, we investigated the influence of magnetite (FeO), troilite (FeS), and silica (SiO) on the microbial diversity, cell viability, biofilm formation, and EPS composition of an oil-recovered multispecies consortium.

METHODS

An oilfield-recovered microbial consortium was grown for 2 weeks in separate bioreactors, each containing 10 g of commercially available magnetite (FeO), troilite (FeS), or silica (SiO) at 40°C ± 1°C under a gas atmosphere of 20% CO/80% N

RESULTS

The microbial population formed in troilite significantly differed from those in silica and magnetite, which exhibited significant similarities. The dominant taxa in troilite was the genus, whereas dominated in magnetite and silica. Nevertheless, biofilm formation was lowest on troilite and highest on silica, correlating with the observed cell viability.

DISCUSSION

The dissolution of troilite followed by the liberation of HS (HS) and Fe into the test solution, along with its larger particle size compared to silica, likely contributed to the observed results. Confocal laser scanning microscopy revealed that the EPS of the biofilm formed in silica was dominated by eDNA, while those in troilite and magnetite primarily contained polysaccharides. Although the mechanisms of this phenomenon could not be determined, these findings are anticipated to be particularly valuable for enhancing MIC mitigation strategies currently used in oil and gas systems.