Biochemical profile of isolates, with a main focus on the fatty acid profile.

Biochemical characterization of new microalgal strains that are isolated from diverse environmental conditions is an important starting point for the establishment of high-quality feedstock for nutraceutical and pharmaceutical applications. In this research study, the biochemical composition of three Iranian native subspecies of microalgae (, , and sp.), with the main focus on fatty acid composition, was studied. The results showed that the strain had the highest biomass productivity (48 g/L/d), biomass (0.73%), carbohydrate (13.97%), fat (16.27%), protein (44.04%), chlorophyll-a (6.32 mg/g), and carotenoids (3.7 mg/g). The lipid profile also revealed that had the highest percentage of polyunsaturated fatty acid (46.52%), ratio of ∑n-3/∑n-6 (5.96), ratio of polyunsaturated fatty acid to saturated fatty acid (PUFA/SAF) (1.18), α-linolenic acid (22.74%), hypocholesterolemia index (1.61), and low atherogenic index (0.34). and , thus, showed a great promise in nutraceutical and pharmaceutical applications due to their appropriate high productivity, biopigment, protein, lipid, antioxidant activity, long-chain polyunsaturated fatty acids, and α-linolenic acid.