Samodien Sedicka, de Kock Maryna, Joubert Elizabeth, de Beer Dalene, Kriel Jurgen, Gelderblom Wentzel C A, Lilly Mariska
Applied Microbial and Health Biotechnology Institute Cape Peninsula University of Technology Bellville South Africa.
Department of Medical Bioscience Program University of Western Cape Bellville South Africa.
Food Sci Nutr. 2024 May 15;12(8):5647-5662. doi: 10.1002/fsn3.4214. eCollection 2024 Aug.
The anti-cancer potential of species (honeybush) has been demonstrated in several models. The present study investigated the effects of aqueous and polyphenol-enriched (PE) extracts of and , as well as mangiferin and hesperidin, on different cell growth parameters in human liver (HepG2) and colon (HT-29) cancer cells. Mangiferin and hesperidin were most abundant in and , respectively. extracts had the highest ferric-reducing antioxidant capacity. Following exposure of the cells to the extracts and compounds, cell viability, proliferation, and death (apoptosis and autophagy) were determined. extracts reduced cell viability and inhibited cell proliferation the most, associated with depletion of ATP. In HepG2 cells, the PE extracts were less effective than the aqueous extracts in reducing cell viability but more effective in inhibiting cell proliferation. Despite disrupting cell growth, none of the extracts induced apoptosis. The aqueous extracts affected autophagy in both cancer cells. Disruption of mitochondrial membrane integrity by the different extracts, presumably via polyphenol/iron interactions, is postulated to be involved; however, mangiferin and hesperidin had no effect, suggesting that other polyphenols and/or complex interactions between compounds are likely responsible for the differential cytotoxic and/or cytoprotective effects of the extracts.
某物种（蜜树茶）的抗癌潜力已在多种模型中得到证实。本研究调查了该物种及另一物种的水提取物和富含多酚（PE）的提取物，以及芒果苷和橙皮苷对人肝癌（HepG2）细胞和结肠癌细胞（HT - 29）不同细胞生长参数的影响。芒果苷和橙皮苷分别在该物种及另一物种中含量最为丰富。该物种提取物具有最高的铁还原抗氧化能力。在细胞暴露于提取物和化合物后，测定细胞活力、增殖和死亡（凋亡和自噬）情况。该物种提取物最能降低细胞活力并抑制细胞增殖，这与ATP的消耗有关。在HepG2细胞中，PE提取物在降低细胞活力方面不如水提取物有效，但在抑制细胞增殖方面更有效。尽管扰乱了细胞生长，但没有一种提取物诱导细胞凋亡。水提取物影响了两种癌细胞的自噬。推测不同提取物通过多酚/铁相互作用破坏线粒体膜完整性，但芒果苷和橙皮苷没有这种作用，这表明其他多酚和/或化合物之间的复杂相互作用可能是提取物产生不同细胞毒性和/或细胞保护作用的原因。
