基于适配体-鲁米诺修饰的磁性氧化石墨烯和铜基金属有机骨架复合的新型化学发光传感器用于甲胎蛋白检测。

A novel chemiluminescence sensor for alpha-fetoprotein detection based on an aptamer-luminol modified magnetic graphene oxide and copper-based MOF composite.

机构信息

Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China.

Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.

出版信息

Anal Methods. 2024 Aug 22;16(33):5723-5732. doi: 10.1039/d4ay01175a.

DOI:10.1039/d4ay01175a

PMID:39140150

Abstract

Herein, an aptamer-luminol modified magnetic graphene oxide and copper-based MOF composite was prepared and used to build a novel target-triggered "turn on" chemiluminescence (CL) sensor for alpha-fetoprotein (AFP) detection. Magnetic graphene oxide (MGO) was functionalized with the complementary sequence of the AFP aptamer (cDNA), and then MGO-cDNA was linked to aptamer modified luminol (Apt-luminol) through the complementary base pairing effect. The functionalized magnetic graphene oxide (MGO-cDNA/Apt-luminol) was prepared as a specific magnetic separation and signal switch material. ZnONPs-Au@CuMOFs shows excellent catalytic performance and was used as a catalyst for the luminol-HO reaction. AFP will specifically recognize and bind to Apt on MGO-cDNA/Apt-luminol when AFP is present, which causes luminol release and triggers the CL reaction. The released luminol encounters ZnONPs-Au@CuMOFs and produces strong CL intensity. Therefore, a novel target-triggered "turn on" CL method with high selectivity and sensitivity for detecting AFP has been established. The linear range and detection limit were 1.0 × 10-50 ng mL and 4.2 × 10 ng mL, respectively. The sensor also exhibited good selectivity, reproducibility and stability, and was finally used for AFP detection in serum samples.

摘要

本文制备了一种适体-鲁米诺修饰的磁性氧化石墨烯和铜基金属有机骨架复合材料，并将其用于构建一种新型的基于靶标触发的“开启”化学发光（CL）传感器，用于检测甲胎蛋白（AFP）。磁性氧化石墨烯（MGO）通过互补序列的 AFP 适体（cDNA）功能化，然后通过互补碱基配对效应将 MGO-cDNA 与适体修饰的鲁米诺（Apt-luminol）连接。功能化的磁性氧化石墨烯（MGO-cDNA/Apt-luminol）被制备为一种特定的磁性分离和信号开关材料。ZnONPs-Au@CuMOFs 表现出优异的催化性能，并被用作鲁米诺-HO 反应的催化剂。当存在 AFP 时，AFP 将特异性识别并结合到 MGO-cDNA/Apt-luminol 上的 Apt，导致鲁米诺释放并触发 CL 反应。释放的鲁米诺遇到 ZnONPs-Au@CuMOFs 并产生强 CL 强度。因此，建立了一种用于检测 AFP 的高选择性和高灵敏度的新型基于靶标触发的“开启”CL 方法。该方法的线性范围和检测限分别为 1.0×10-50 ng mL 和 4.2×10 ng mL。该传感器还表现出良好的选择性、重现性和稳定性，最终用于血清样品中 AFP 的检测。