Liaoning Key Laboratory of Plant Pathology, College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
Rice Research Institute, College of Agriculture, Shenyang Agricultural University, Shenyang, China.
Physiol Plant. 2024 Jul-Aug;176(4):e14475. doi: 10.1111/ppl.14475.
Rhizoctonia solani is a fungal pathogen that causes significant losses in agricultural production. Because of its rapid transmission and broad host range, the exploration of genes involved in defense responses to the infection of R. solani has become an important task. Here, we performed a time-course RNA-Seq experiment to explore crucial genes or pathways involved in host responses to R. solani AG3-TB infection at 6, 12, 24, 36, 48, and 72 hours post inoculation (hpi). GO and KEGG enrichment analysis revealed that most DEGs were enriched in the basal metabolism pathways, including carbohydrate metabolic processes and the biosynthesis of amino acids. Moreover, catalase (CAT) and superoxide dismutase (SOD) were up-regulated, and transcription factors (TFs) such as WRKY, AP2, and MYB were increased significantly compared to the control (0 hpi). Silencing of WRKY70 and catalase-3 exhibited elevated susceptibility to the fungal infection. To summarize, the TFs WRKY70 and WRKY75, genes involved in jasmonic acid (JA), salicylic acid (SA), and brassinosteroids (BR) signaling pathways, and defense-related enzymes may play crucial roles in the host responses to R. solani AG3-TB infection.
茄丝核菌是一种真菌病原体，它会导致农业生产的重大损失。由于其快速传播和广泛的宿主范围，探索与茄丝核菌感染防御反应相关的基因已成为一项重要任务。在这里，我们进行了一个时间进程 RNA-Seq 实验，以探索在接种后 6、12、24、36、48 和 72 小时（hpi）时宿主对茄丝核菌 AG3-TB 感染的反应中涉及的关键基因或途径。GO 和 KEGG 富集分析表明，大多数差异表达基因富集在基础代谢途径中，包括碳水化合物代谢过程和氨基酸的生物合成。此外，与对照（0 hpi）相比，过氧化氢酶 (CAT) 和超氧化物歧化酶 (SOD) 上调，转录因子 (TF) 如 WRKY、AP2 和 MYB 显著增加。WRKY70 和过氧化氢酶-3 的沉默表现出对真菌感染的敏感性增加。总之，WRKY70 和 WRKY75 这两个 TF，以及涉及茉莉酸 (JA)、水杨酸 (SA) 和油菜素内酯 (BR) 信号通路和防御相关酶的基因，可能在宿主对茄丝核菌 AG3-TB 感染的反应中发挥关键作用。
