Beijing Institute of Ophthalmology, Beijing Tongren Hospital, Capital Medical University.
Beijing Institute of Ophthalmology, Beijing Tongren Hospital, Capital Medical University;
J Vis Exp. 2024 Jul 26(209). doi: 10.3791/67016.
Due to the limited accessibility of the human retina, retinal organoids (ROs) are the best model for studying human retinal disease, which could reveal the mechanism of retinal development and the occurrence of retinal disease. Microglia (MG) are unique resident macrophages in the retina and central nervous system (CNS), serving crucial immunity functions. However, retinal organoids lack microglia since their differentiation origin is the yolk sac. The specific pathogenesis of microglia in these retinal diseases remains unclear; therefore, the establishment of a microglia-incorporated retinal organoid model turns out to be necessary. Here, we successfully constructed a co-cultured model of retinal organoids with microglia derived from human stem cells. In this article, we differentiated microglia and then co-cultured to retinal organoids in the early stage. As the incorporation of immune cells, this model provides an optimized platform for retinal disease modeling and drug screening to facilitate in-depth research on the pathogenesis and treatment of retinal and CNS-related diseases.
由于人视网膜的有限可及性，视网膜类器官（RO）是研究人类视网膜疾病的最佳模型，它可以揭示视网膜发育和视网膜疾病发生的机制。小胶质细胞（MG）是视网膜和中枢神经系统（CNS）中独特的常驻巨噬细胞，发挥着至关重要的免疫功能。然而，由于视网膜类器官的分化起源于卵黄囊，因此缺乏小胶质细胞。这些视网膜疾病中小胶质细胞的特定发病机制仍不清楚；因此，建立包含小胶质细胞的视网膜类器官模型是必要的。在这里，我们成功构建了一种源自人类干细胞的小胶质细胞与视网膜类器官共培养模型。在本文中，我们从小胶质细胞分化开始，然后在早期阶段与视网膜类器官共培养。作为免疫细胞的掺入，该模型为视网膜疾病建模和药物筛选提供了一个优化的平台，有助于深入研究视网膜和 CNS 相关疾病的发病机制和治疗方法。
