Bioengineering Laboratory, Department of Textile and Fibre Engineering, Indian Institute of Technology, New Delhi 110016, India.
Bioengineering Laboratory, Department of Textile and Fibre Engineering, Indian Institute of Technology, New Delhi 110016, India.
Int J Biol Macromol. 2024 Oct;278(Pt 1):134693. doi: 10.1016/j.ijbiomac.2024.134693. Epub 2024 Aug 12.
It aims to prepare the chitosan (CS) and polyethylene oxide (PEO) hydrogel membranes with different CS/PEO blend ratios (100:0, 95:5, 90:10, 80:20 and 70:30) via solvent casting. The physicochemical properties of these membranes were investigated using various characterization techniques: Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), energy dispersive X-ray (EDX), contact angle, and tensile testing. The interaction of PEO and chitosan was investigated by DSC in terms of freezing bound, freezing free, and non-freezing PEO fraction. The cross-sectional surface morphology of membranes displayed a smoother surface with increasing PEO content up to 20 %, beyond which nonhomogeneity on the surface was visible. The antifouling behavior of membranes was investigated by bacterial adherence study, which showed an enhanced antifouling nature of membranes with the increase in the PEO content. The peeling strength of the membranes was measured using a 90° angle peeling test, and it was found that 20 % and more PEO content promotes easy removal from the gelatin slab. In addition to this, live/ dead assay of the CS was performed to visualize the presence of live and dead bacteria on the surface. The CS/PEO blend with 20 % PEO content has properties makes it suitable for use as a protective layer on wound dressings to prevent bacterial growth. It's use in wound dressings has the potential to reduce the pain during the time of dressing removal and improve patient outcomes. The present investigation leads to the development of a CS hydrogel matrix which exhibits very interesting interaction with the PEO moiety along with its innovative feature of antifouling and antimicrobial nature.
它旨在通过溶剂浇铸法制备不同壳聚糖（CS）/聚乙烯氧化物（PEO）共混比（100:0、95:5、90:10、80:20 和 70:30）的 CS/PEO 水凝胶膜。使用各种特性化技术研究这些膜的物理化学性质：傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）、差示扫描量热法（DSC）、扫描电子显微镜（SEM）、原子力显微镜（AFM）、能量色散 X 射线（EDX）、接触角和拉伸测试。通过 DSC 研究 PEO 和壳聚糖的相互作用，涉及冻结结合、冻结自由和非冻结 PEO 分数。膜的横截面表面形态显示出更光滑的表面，随着 PEO 含量的增加，直到 20%，超过该值，表面出现不均匀性。通过细菌附着研究研究了膜的抗污染行为，结果表明随着 PEO 含量的增加，膜的抗污染性质得到增强。使用 90°角剥离试验测量膜的剥离强度，发现 20%及以上的 PEO 含量促进从明胶板上轻松去除。除此之外，还对 CS 进行了活/死测定，以观察表面上活细菌和死细菌的存在。具有 20%PEO 含量的 CS/PEO 共混物具有使其适合用作伤口敷料的保护层以防止细菌生长的特性。它在伤口敷料中的应用有可能减少敷料去除过程中的疼痛并改善患者的结果。本研究导致开发了一种 CS 水凝胶基质，该基质与 PEO 部分表现出非常有趣的相互作用，同时具有抗污染和抗菌的创新特性。
