Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of Education, Laboratory of Biochemistry, College of Chemistry, Xiangtan University, Xiangtan, 411105, China.
Food Chem Toxicol. 2024 Oct;192:114931. doi: 10.1016/j.fct.2024.114931. Epub 2024 Aug 12.
Oral exposure to nanoparticles (NPs) may affect intestinal microbiota, and this effect may be further changed by co-contaminates. In the present study, we investigated the combined effects of TiO NPs and fipronil (FPN) on microbiota in mouse intestines. Mice were intragastric exposed to 5.74 mg/kg TiO NPs, 2.5 mg/kg FPN, or both of them, once a day, for 30 days. The results showed that individual exposure to TiO NPs or FPN decreased body weight and induced pathological changes in intestines. The exposure was also associated with increased cleaved caspase-3 protein, oxidative stress and decreased tight junction protein expression. Furthermore, the levels of diamine oxidase (DAO), lipopolysaccharide (LPS) and inflammatory cytokines in serum were also elevated, indicating increased intestinal barrier permeability. As expected, both TiO NPs and FPN decreased the diversity and altered the composition of microbiota. However, the observed effects were not further enhanced after the co-exposure to TiO NPs and FPN, except that Romboutsia was only significantly increased after the co-exposure to TiO NPs + FPN. We concluded that oral exposure to TiO NPs and FPN showed minimal synergistic effects on microbiota in mouse intestine.
经口暴露于纳米颗粒（NPs）可能会影响肠道微生物群，而这种影响可能会被共污染物进一步改变。在本研究中，我们研究了 TiO NPs 和氟虫腈（FPN）对小鼠肠道微生物群的联合作用。小鼠每天经胃内暴露于 5.74mg/kg 的 TiO NPs、2.5mg/kg 的 FPN 或两者的混合物，持续 30 天。结果表明，单独暴露于 TiO NPs 或 FPN 会降低体重并引起肠道的病理变化。这种暴露还与增加的半胱天冬酶-3 蛋白、氧化应激和紧密连接蛋白表达减少有关。此外，血清中二胺氧化酶（DAO）、脂多糖（LPS）和炎症细胞因子的水平也升高，表明肠道屏障通透性增加。正如预期的那样，TiO NPs 和 FPN 均降低了微生物群的多样性并改变了其组成。然而，在 TiO NPs 和 FPN 共暴露后，并未观察到观察到协同作用进一步增强，除了共暴露于 TiO NPs+FPN 后罗蒙氏菌显著增加。我们得出结论，经口暴露于 TiO NPs 和 FPN 对小鼠肠道微生物群的协同作用最小。
