College of Food and Health, Zhejiang A&F University, Hangzhou 311300, China.
College of Optical, Mechanical and Electrical Engineering, Zhejiang A&F University, Hangzhou 311300, China.
Anal Chim Acta. 2024 Sep 1;1320:342999. doi: 10.1016/j.aca.2024.342999. Epub 2024 Jul 22.
Fluoroquinolones (FQs) are widely used for their excellent antimicrobial properties, yet their release into aquatic environments pose risks to ecosystems and public health. The accurate monitoring and analysis of FQs present challenges due to their low concentrations and the complex matrices found in actual environmental samples. To address the need for auto-pretreatment and on-line instrumental analysis, developing new microextraction materials and protocols is crucial. Such advancements will provide better analytical assurance for the effective extraction and determination of FQs at trace levels, which is of great significance to environmental protection and human health.
In this work, we presented a Co mediated paper-based molecularly imprinted polymer chip (CMC@Co-MIP), combined with UPLC analysis, to develop an effective analytical method for identifying and quantifying trace amounts of ciprofloxacin (CIP) and enrofloxacin (ENR) in water samples. Notably, the addition of Co in CMC@Co-MIP helped to capture the template molecule CIP through coordination before imprinting, which significantly improved the ordering of the imprinted cavities. CMC@Co-MIP exhibited a maximum adsorption capacity up to 500.20 mg g with an imprinting factor of 4.12, surpassing previous reports by a significant margin. Furthermore, the enrichment mechanism was extensively analyzed by various characterization techniques. The developed method showed excellent repeatability and reproducibility (RSD < 13.0 %) with detection limits ranging from 0.15 to 0.21 μg L and recoveries ranging from 64.9 % to 102.3 % in real spiked water samples.
We developed a novel microextraction paper-based chip based on Co mediation, which effectively improved the selectivity and convenience of extracting FQs. This breakthrough allowed the chip to have a high enrichment efficiency as well as provide a robust on-line instrumental program. It also confirms that the imprinting scheme based on metal ion coordination is a high-performance strategy.
氟喹诺酮类（FQs）因其出色的抗菌特性而被广泛应用，但它们在环境中的释放对生态系统和公共健康构成了风险。由于其浓度低且实际环境样品中存在复杂基质，因此对 FQs 的准确监测和分析具有挑战性。为了实现自动预处理和在线仪器分析，开发新的微萃取材料和方案至关重要。这些进展将为痕量 FQs 的有效提取和测定提供更好的分析保证，这对于环境保护和人类健康具有重要意义。
在这项工作中，我们提出了一种 Co 介导的纸基分子印迹聚合物芯片（CMC@Co-MIP），结合 UPLC 分析，开发了一种有效分析方法，用于识别和定量水样中的痕量环丙沙星（CIP）和恩诺沙星（ENR）。值得注意的是，在印迹前，Co 的加入有助于通过配位捕获模板分子 CIP，从而显著改善印迹腔的有序性。CMC@Co-MIP 表现出高达 500.20 mg/g 的最大吸附容量和 4.12 的印迹因子，显著优于先前的报道。此外，通过各种特征化技术广泛分析了富集机制。该方法在实际加标水样中表现出出色的重复性和再现性（RSD <13.0%），检测限范围为 0.15 至 0.21 μg/L，回收率范围为 64.9%至 102.3%。
我们开发了一种基于 Co 介导的新型微萃取纸基芯片，有效地提高了提取 FQs 的选择性和便利性。这一突破使芯片具有高富集效率，并提供了强大的在线仪器程序。它还证实了基于金属离子配位的印迹方案是一种高性能策略。
