Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan Province 650500, China.
Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan Province 650500, China; Yunnan Agricultural University, Kunming, 650201, China.
Anal Chim Acta. 2024 Sep 1;1320:343014. doi: 10.1016/j.aca.2024.343014. Epub 2024 Jul 27.
Herein, the selenium (Se) modified gold nanoparticles (Se-AuNPs) was synthesized using cerium doped carbon dots (Ce-CDs) as a reducing agent and template. As desired, Se-AuNPs displays enhanced peroxidase (POD)-like activity in the presence of Hg. The mechanism for the enhanced activity was attributed to the increased affinity between Se-AuNPs-Hg and the substrate, in which Se and Au elements have a strong binding capacity to Hg, forming Hg-Se bonds and Au-Hg amalgam to generate more ·OH. This POD-like activity of Se-AuNPs-Hg correlates with the colorimetric reaction by the catalytic reaction between 3,3',5,5'-tetramethylbenzidine (TMB) and HO. The oxidation of TMB was completely inhibited by the introduction of the reductive S. Based on the above findings, a strategy for the colorimetric detection of Hg and S by Se-AuNPs was established with linear ranges of 0.33-66 μg/L and 0.625-75 μg/L, and low detection limits of 0.17 μg/L and 0.12 μg/L (3.3 δ/k), respectively. When the colorimetric probes for detection of Hg and S was applied in environmental water samples, the recoveries were in the range of 90.3-108.0 %. This method will provide a new idea for the colorimetric detection strategy of Hg due to the strong interaction between Hg and Se.
在此，使用铈掺杂碳点 (Ce-CDs) 作为还原剂和模板合成了硒 (Se) 修饰的金纳米粒子 (Se-AuNPs)。如预期的那样，在存在 Hg 的情况下，Se-AuNPs 显示出增强的过氧化物酶 (POD) 样活性。增强活性的机制归因于 Se-AuNPs-Hg 与底物之间增加的亲和力，其中 Se 和 Au 元素对 Hg 具有很强的结合能力，形成 Hg-Se 键和 Au-Hg 汞齐，从而产生更多的 ·OH。这种 Se-AuNPs-Hg 的 POD 样活性与通过 3,3',5,5'-四甲基联苯胺 (TMB) 和 HO 之间的催化反应的比色反应相关。引入还原剂 S 完全抑制了 TMB 的氧化。基于上述发现，建立了一种基于 Se-AuNPs 的比色法检测 Hg 和 S 的策略，其线性范围分别为 0.33-66 μg/L 和 0.625-75 μg/L，检测限分别为 0.17 μg/L 和 0.12 μg/L（3.3 δ/k）。当用于检测 Hg 和 S 的比色探针应用于环境水样时，回收率在 90.3-108.0% 的范围内。由于 Hg 和 Se 之间的强相互作用，该方法将为 Hg 的比色检测策略提供新的思路。
