Analysis and Test Center, Chinese Academy of Tropical Agricultural Sciences, Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables, Key Laboratory of Quality and Safety Control of Subtropical Fruits and Vegetables, Ministry of Agriculture and Rural Affairs, Haikou, China.
School of Food Science and Engineering, Hainan University, Haikou, China.
Luminescence. 2024 Aug;39(8):e4871. doi: 10.1002/bio.4871.
Graphene oxide (GO) and copper nanoparticles (Cu NPs) were incorporated to modulate and enhance the fluorescence properties of pegylated graphite phase carbon nitride (g-CN-PEG). Combined with the specific recognition capability of a molecular imprinted polymer (MIP), a highly sensitive and selective fluorescent molecular imprinted probe for dopamine detection was developed. The fluorescent g-CN-PEG was synthesized from melamine and modified with GO and Cu NPs to obtain GO/g-CN-PEG@Cu NPs. Subsequently, MIP was prepared on the surface of GO/g-CN-PEG@Cu NPs using dopamine as the template molecule. Upon elution of the template molecule, a dopamine-specific GO/g-CN-PEG@Cu NPs/MIP fluorescence probe was obtained. The fluorescence intensity of the probe was quenched through the adsorption of different concentrations of dopamine by the MIP, thus establishing a novel method for the detection of dopamine. The linear range of dopamine detection was from 5 × 10 to 6 × 10 mol L, with a detection limit of 2.32 × 10 mol L. The sensor was utilised for the detection of dopamine in bananas, achieving a spiked recovery rate between 90.3% and 101.3%. These results demonstrate that the fluorescence molecular imprinted sensor developed in this study offers a highly sensitive approach for dopamine detection in bananas.
氧化石墨烯（GO）和铜纳米粒子（Cu NPs）被掺入到聚乙二醇化石墨相氮化碳（g-CN-PEG）中，以调节和增强其荧光性质。结合分子印迹聚合物（MIP）的特异性识别能力，开发了一种用于多巴胺检测的高灵敏、高选择性荧光分子印迹探针。荧光 g-CN-PEG 是由三聚氰胺合成的，并经过 GO 和 Cu NPs 的修饰，得到 GO/g-CN-PEG@Cu NPs。随后，以多巴胺为模板分子，在 GO/g-CN-PEG@Cu NPs 表面制备 MIP。模板分子洗脱后，得到多巴胺特异性的 GO/g-CN-PEG@Cu NPs/MIP 荧光探针。探针的荧光强度通过 MIP 对不同浓度多巴胺的吸附而被猝灭，从而建立了一种用于检测多巴胺的新方法。多巴胺检测的线性范围为 5×10 到 6×10 mol L，检测限为 2.32×10 mol L。该传感器用于检测香蕉中的多巴胺，实际样品的加标回收率在 90.3%到 101.3%之间。这些结果表明，本研究开发的荧光分子印迹传感器为香蕉中多巴胺的检测提供了一种高灵敏度的方法。
