Laboratory of Microorganisms and Active Biomolecules (LR03ES03), Faculty of Sciences of Tunis, University of Tunis El Manar, Campus Universitaire 2092, Tunis, Tunisia.
Neurology Department, Razi University Hospital, Manouba, Tunisia.
Curr Alzheimer Res. 2024;21(4):232-241. doi: 10.2174/0115672050327009240808103542.
Alzheimer's disease (AD) is the most prevalent neurodegenerative disorder, with a significant burden on global health. AD is characterized by a progressive cognitive decline and memory loss. Emerging research suggests a potential link between periodontitis, specifically the presence of oral bacteria such as (), and AD progression. produces an enzyme, Agmatine deiminase (AgD), which converts agmatine to N-carbamoyl putrescine (NCP), serving as a precursor to essential polyamines. Recent studies have confirmed the correlation between disruptions in polyamine metabolism and cognitive impairment.
This study aims to investigate the dysregulation of Agmatine deiminase () in the context of AD.
Saliva samples were collected from a total of 54 individuals, including 27 AD patients and 27 healthy controls. The expression of the gene was analyzed using quantitative Real-- Time PCR.
The results showed a significant decrease in gene expression in the saliva samples of AD patients compared to healthy controls. This downregulation was found in AD patients with advanced stages of periodontitis. Additionally, a correlation was observed between the decrease in expression and the 30-item Mini-Mental State Examination (MMSE) score.
These findings suggest that measuring expression in saliva could be a noninvasive tool for monitoring AD progression and aid in the early diagnosis of patients with periodontitis. Further research is needed to validate our results and explore the underlying mechanisms linking periodontitis, expression, and AD pathophysiology.
阿尔茨海默病（AD）是最常见的神经退行性疾病，对全球健康造成了重大负担。AD 的特征是认知能力逐渐下降和记忆力丧失。新的研究表明，牙周炎（尤其是口腔细菌如 ）与 AD 进展之间存在潜在联系。 产生一种酶，胍氨酸脱亚氨酶（AgD），它将胍氨酸转化为 N-氨甲酰腐胺（NCP），作为必需多胺的前体。最近的研究证实了多胺代谢紊乱与认知障碍之间的相关性。
本研究旨在探讨 AD 背景下 胍氨酸脱亚氨酶（）的失调。
共采集了 54 人的唾液样本，其中包括 27 名 AD 患者和 27 名健康对照者。采用定量实时 PCR 分析 基因的表达。
结果显示，与健康对照组相比，AD 患者唾液样本中的 基因表达显著下降。在患有晚期牙周炎的 AD 患者中发现了这种下调。此外，还观察到 表达下降与 30 项简易精神状态检查（MMSE）评分之间存在相关性。
这些发现表明，测量唾液中的 表达可能是监测 AD 进展的非侵入性工具，并有助于早期诊断患有牙周炎的患者。需要进一步的研究来验证我们的结果，并探讨牙周炎、 表达与 AD 病理生理学之间的潜在机制。
