Wang Xiaoyu, Yang Shuai, Gao Qiang, Dai Youqing, Tian Lei, Wen Liang, Yan Honghao, Yang Long, Hou Xin, Liu Peng, Zhang Li
College of Plant Protection, Shandong Agricultural University, Tai'an, China.
Yuxi Zhongyan Tobacco Seed Co., Ltd., Yu'xi, China.
Front Microbiol. 2024 Jul 31;15:1436382. doi: 10.3389/fmicb.2024.1436382. eCollection 2024.
The quality of fermented plant leaves is closely related to the interleaf microorganisms and their metabolic activities. In this experiment, a multi-omics analysis was applied to investigate the link between the structural composition of the phyllosphere microbial community and the main metabolites during the fermentation process. It was found that the whole fermentation process of cigar leaves could be divided into three stages, in which the Mid-Stage was the most active period of microbial metabolic activities and occupied an important position. and played important roles in this fermentation. and are the microorganisms that play an important role in the fermentation process. were strongly correlated with lipids and amino acids, despite its low abundance, is importantly associated with terpene and plays a significant role throughout the process. It is worth noting that Wapper exists more characteristic fungal genera than Filler and is more rapid in fermentation progress, which implies that the details of the fermentation process should be adjusted appropriately to ensure stable quality when faced with plant leaves of different genotypes. This experiment explored the relationship between metabolites and microorganisms, and provided a theoretical basis for further optimizing the fermentation process of plant leaves and developing techniques to improve product quality. Biomarker is mostly present in the pre-fermentation phase, but the mid-fermentation phase is the most important part of the process.
发酵植物叶片的品质与叶际微生物及其代谢活动密切相关。在本实验中，采用多组学分析方法研究叶际微生物群落结构组成与发酵过程中主要代谢产物之间的联系。结果发现，雪茄烟叶的整个发酵过程可分为三个阶段，其中中期是微生物代谢活动最活跃的时期，占据重要地位。[此处原文两个“and”，疑有信息缺失，暂按原文翻译]在该发酵过程中发挥重要作用。[此处原文两个“and”，疑有信息缺失，暂按原文翻译]是在发酵过程中起重要作用的微生物。[此处原文两个“and”，疑有信息缺失，暂按原文翻译]与脂质和氨基酸呈强相关，尽管其丰度较低，但[此处原文“ is”疑有误，暂按原文翻译]与萜烯密切相关且在整个过程中发挥重要作用。值得注意的是，外包叶比内包叶存在更多的特征真菌属，且发酵进程更快，这意味着面对不同基因型的植物叶片时，应适当调整发酵过程的细节以确保品质稳定。本实验探究了代谢产物与微生物之间的关系，为进一步优化植物叶片发酵工艺及开发提高产品品质的技术提供了理论依据。生物标志物大多存在于发酵前期，但发酵中期是该过程最重要的部分。
