Cardenas Roberto Bravo, Watson Clifford, Valentin-Blasini Liza
Centers for Disease Control and Prevention, Tobacco Volatiles Branch, Atlanta, GA 30341, USA.
Contrib Tob Nicotine Res. 2021 Nov;30(4):212-220. doi: 10.2478/cttr-2021-0016.
The new pod devices like JUUL, Vuse Alto, myblu, and other ?pod-mod" related products had a huge impact on the e-cigarette market, especially among teens and young adults due in particular to aggressive marketing on social media, wide availability, and discrete use due to their special design. These pod devices are designed to deliver nicotine levels per puff comparable to combustible cigarettes while producing smaller amounts of visible exhaled aerosol from the heating of e-liquids. Some of these liquids contain high concentrations of acids, such as benzoic acid, to allow higher nicotine deliveries with less harshness and throat irritation. Benzoic acid is a potential source of the human carcinogen benzene and a chemical of concern. Besides acids, flavoring agents such as benzyl alcohol, a local anesthetic that could facilitate tobacco smoke inhalation are also common in these devices. Both benzoic acid and benzyl alcohol in e-liquids might be of relevance for the health risk of vapers. An isotope dilution high-performance liquid chromatography-tandem mass spectrometry (ID-HPLC-MS/MS) method has been developed for the detection of benzoic acid and benzyl alcohol in the JUUL brand e-liquids. The sample preparation consisted of a simple dilution followed by a mechanical stirring process. ID-HPLC-MS/MS was used to separate, identify, and quantify the benzoic acid and/or benzyl alcohol in diluted extracts. Detection limits were 0.11 and 9.05 ng/μL for benzyl alcohol and benzoic acid, respectively. Product variability estimated from the analysis of seven different e-liquids in triplicates (n = 21) yielded relative standard deviations ranging from 4.3% to 16.0% for benzyl alcohol and 6.3% to 11.1% for benzoic acid. The amount of benzoic acid (32.8 ± 2.8 mg/g; 3.3 ± 0.3%, w/w) and the nicotine-benzoic acid molar ratio (1.15 ± 0.02) remained relatively consistent among pod flavors. [Contrib. Tob. Nicotine Res. 30 (2021) 212-220].
像JUUL、Vuse Alto、myblu等新型烟弹设备以及其他与“烟弹模组”相关的产品对电子烟市场产生了巨大影响，尤其是在青少年和年轻人当中，这主要归因于社交媒体上的激进营销、广泛可得性以及因其特殊设计而便于 discreet 使用。这些烟弹设备的设计目的是使每口吸入的尼古丁含量与可燃香烟相当，同时在加热电子烟液时产生较少的可见呼出气溶胶。其中一些电子烟液含有高浓度的酸，如苯甲酸，以便在减少刺激性和喉咙不适的情况下实现更高的尼古丁输送量。苯甲酸是一种潜在的人类致癌物苯的来源，也是一种值得关注的化学物质。除了酸之外，调味剂如苄醇（一种可能有助于吸入烟草烟雾的局部麻醉剂）在这些设备中也很常见。电子烟液中的苯甲酸和苄醇都可能与电子烟使用者的健康风险相关。已经开发出一种同位素稀释高效液相色谱 - 串联质谱法（ID - HPLC - MS/MS）用于检测JUUL品牌电子烟液中的苯甲酸和苄醇。样品制备包括简单稀释，然后进行机械搅拌过程。ID - HPLC - MS/MS用于分离、鉴定和定量稀释提取物中的苯甲酸和/或苄醇。苄醇和苯甲酸的检测限分别为0.11和9.05 ng/μL。对七种不同电子烟液进行一式三份分析（n = 21）得出的产品变异性显示，苄醇的相对标准偏差范围为4.3%至16.0%，苯甲酸为6.3%至11.1%。在不同烟弹口味中，苯甲酸的含量（32.8 ± 2.8 mg/g；3.3 ± 0.3%，w/w）和尼古丁 - 苯甲酸摩尔比（1.15 ± 0.02）保持相对一致。[《烟草与尼古丁研究贡献》30（2021）212 - 220] 。 （注：discreet 原意为“谨慎的、不引人注意的”，这里结合语境推测为便于隐秘使用，但中文无完全对应词汇，暂保留英文）
