Determination of Benzoic Acid and Benzyl Alcohol in E-Liquids (JUUL Pods) by Isotopic Dilution High-Performance Liquid Chromatography and Tandem Mass Spectrometry.

The new pod devices like JUUL, Vuse Alto, myblu, and other ?pod-mod" related products had a huge impact on the e-cigarette market, especially among teens and young adults due in particular to aggressive marketing on social media, wide availability, and discrete use due to their special design. These pod devices are designed to deliver nicotine levels per puff comparable to combustible cigarettes while producing smaller amounts of visible exhaled aerosol from the heating of e-liquids. Some of these liquids contain high concentrations of acids, such as benzoic acid, to allow higher nicotine deliveries with less harshness and throat irritation. Benzoic acid is a potential source of the human carcinogen benzene and a chemical of concern. Besides acids, flavoring agents such as benzyl alcohol, a local anesthetic that could facilitate tobacco smoke inhalation are also common in these devices. Both benzoic acid and benzyl alcohol in e-liquids might be of relevance for the health risk of vapers. An isotope dilution high-performance liquid chromatography-tandem mass spectrometry (ID-HPLC-MS/MS) method has been developed for the detection of benzoic acid and benzyl alcohol in the JUUL brand e-liquids. The sample preparation consisted of a simple dilution followed by a mechanical stirring process. ID-HPLC-MS/MS was used to separate, identify, and quantify the benzoic acid and/or benzyl alcohol in diluted extracts. Detection limits were 0.11 and 9.05 ng/μL for benzyl alcohol and benzoic acid, respectively. Product variability estimated from the analysis of seven different e-liquids in triplicates (n = 21) yielded relative standard deviations ranging from 4.3% to 16.0% for benzyl alcohol and 6.3% to 11.1% for benzoic acid. The amount of benzoic acid (32.8 ± 2.8 mg/g; 3.3 ± 0.3%, w/w) and the nicotine-benzoic acid molar ratio (1.15 ± 0.02) remained relatively consistent among pod flavors. [Contrib. Tob. Nicotine Res. 30 (2021) 212-220].