Salters T, Sharpe B, Raychoudhury S S, Nagdas S K
Department of Chemistry, Physics & Materials Science, Fayetteville State University, USA.
Department of Biology, Chemistry and Environmental Health Science, Benedict College, USA.
Int J Biochem Physiol. 2023;8(2). doi: 10.23880/ijbp-16000237. Epub 2023 Oct 21.
Epididymis plays a vital role in promoting sperm maturation and maintaining sperm viability. It has been shown the presence of nonviable sperm in cauda epididymis. We previously identified a secretory protein (260/280KDa oligomers) of hamster cauda epididymal principal cells that binds to nonviable sperm. The 260/280KDa oligomers are composed of 64kDa FGL2 (fibrinogen-like protein-2) and 33kDa FGL1) (fibrinogen-like protein-1). In addition, we have demonstrated that FGL2 is a phospholipid-activated serine protease; the conversion of prothrombin to thrombin by FGL2 followed by the conversion of soluble fibrinogen to insoluble fibrin polymers by thrombin. In the present study, we have shown the presence of a 56kDa fibrinogen β in hamster cauda sperm. The potential role of fibrinogen in hamster physiology is being discussed.
附睾在促进精子成熟和维持精子活力方面发挥着至关重要的作用。已有研究表明附睾尾部存在无活力精子。我们之前鉴定出仓鼠附睾尾部主细胞的一种分泌蛋白（260/280KDa寡聚体），它能与无活力精子结合。260/280KDa寡聚体由64kDa的FGL2（纤维蛋白原样蛋白-2）和33kDa的FGL1（纤维蛋白原样蛋白-1）组成。此外，我们还证明FGL2是一种磷脂激活的丝氨酸蛋白酶；FGL2将凝血酶原转化为凝血酶，随后凝血酶将可溶性纤维蛋白原转化为不溶性纤维蛋白聚合物。在本研究中，我们发现仓鼠附睾尾部精子中存在56kDa的纤维蛋白原β。本文正在讨论纤维蛋白原在仓鼠生理学中的潜在作用。
