One Health Microbiology Laboratory, Postgraduate Program in Medical Microbiology, Department of Pathology and Legal Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo, 1315 - Rodolfo Teófilo - CEP: 60430-275, Fortaleza, Ceará, Brazil.
Division of Glycoscience, Department of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, 10691 Stockholm, Sweden.
J Med Microbiol. 2024 Aug;73(8). doi: 10.1099/jmm.0.001870.
Sporotrichosis is a subcutaneous infection caused by dimorphic species embedded in the clinical clade. Fungi have virulence factors, such as biofilm and melanin production, which contribute to their survival and are related to the increase in the number of cases of therapeutic failure, making it necessary to search for new options. Proton pump inhibitors (PPIs) have already been shown to inhibit the growth and melanogenesis of other fungi. Therefore, this study aimed to evaluate the effect of the PPIs omeprazole (OMP), rabeprazole (RBP), esomeprazole, pantoprazole and lansoprazole on the susceptibility and melanogenesis of species, and their interactions with itraconazole, terbinafine and amphotericin B. The antifungal activity of PPIs was evaluated using the microdilution method, and the combination of PPIs with itraconazole, terbinafine and amphotericin B was assessed using the checkerboard method. The assessment of melanogenesis inhibition was assessed using grey scale. The OMP and RBP showed significant MIC results ranging from 32 to 256 µg ml and 32 to 128 µg ml, respectively. Biofilms were sensitive, with a significant reduction (<0.05) in metabolic activity of 52% for OMP and 50% for RBP at a concentration of 512 µg ml and of biomass by 53% for OMP and 51% for RBP at concentrations of 512 µg ml. As for the inhibition of melanogenesis, only OMP showed inhibition, with a 54% reduction. It concludes that the PPIs OMP and RBP have antifungal activity against planktonic cells and biofilms of species and that, in addition, OMP can inhibit the melanization process in species.
孢子丝菌病是一种由临床枝孢属的二态性种引起的皮下感染。真菌具有毒力因子，如生物膜和黑色素的产生，这有助于它们的生存，并与治疗失败病例的增加有关，因此有必要寻找新的选择。质子泵抑制剂 (PPIs) 已被证明可以抑制其他真菌的生长和黑色素生成。因此，本研究旨在评估质子泵抑制剂奥美拉唑 (OMP)、雷贝拉唑 (RBP)、埃索美拉唑、泮托拉唑和兰索拉唑对 种的敏感性和黑色素生成的影响，以及它们与伊曲康唑、特比萘芬和两性霉素 B 的相互作用。使用微量稀释法评估 PPIs 的抗真菌活性，使用棋盘法评估 PPIs 与伊曲康唑、特比萘芬和两性霉素 B 的联合作用。使用灰度评估黑色素生成抑制的评估。OMP 和 RBP 显示出显著的 MIC 结果，范围分别为 32 至 256 µg/ml 和 32 至 128 µg/ml。生物膜敏感，OMP 和 RBP 在 512 µg/ml 浓度下代谢活性分别显著降低 (<0.05)52%和 50%，生物量分别降低 53%和 51%。至于黑色素生成的抑制，只有 OMP 显示出抑制作用，抑制率为 54%。结论是，PPIs OMP 和 RBP 对 种的浮游细胞和生物膜具有抗真菌活性，此外，OMP 可以抑制 种的黑色素化过程。
