The National Key Engineering Lab of Crop Stress Resistance Breeding, The School of Life Sciences, Anhui Agricultural University, Hefei, China.
Center for Crop Pest Detection and Control, Anhui Agricultural University, Hefei, China.
Plant Biotechnol J. 2024 Dec;22(12):3326-3341. doi: 10.1111/pbi.14451. Epub 2024 Aug 15.
Corn leaf aphids (Rhopalosiphum maidis) are highly destructive pests of maize (Zea mays) that threaten growth and seed yield, but resources for aphid resistance are scarce. Here, we identified an aphid-resistant maize mutant, resistance to aphids 1 (rta1), which is allelic to LIGULELESS1 (LG1). We confirmed LG1's role in aphid resistance using the independent allele lg1-2, allelism tests and LG1 overexpression lines. LG1 interacts with, and increases the stability of ZINC-FINGER PROTEIN EXPRESSED IN INFLORESCENCE MERISTEM (ZIM1), a central component of the jasmonic acid (JA) signalling pathway, by disturbing its interaction with the F-box protein CORONATINE INSENSITIVE 1a (COI1a). Natural variation in the LG1 promoter was associated with aphid resistance among inbred lines. Moreover, a loss-of-function mutant in the LG1-related gene SPL8 in the dicot Arabidopsis thaliana conferred aphid resistance. This study revealed the aphid resistance mechanism of lg1, providing a theoretical basis and germplasm for breeding aphid-resistant crops.
玉米叶蝉（Rhopalosiphum maidis）是玉米（Zea mays）的一种极具破坏性的害虫，会威胁玉米的生长和种子产量，但抗虫资源却很匮乏。在这里，我们鉴定出了一个抗叶蝉的玉米突变体，抗叶蝉 1（rta1），它与 LEAFY GLUME 1（LG1）等位。我们利用独立的等位基因 lg1-2、等位基因测试和 LG1 过表达系证实了 LG1 在抗虫中的作用。LG1 通过干扰其与 F-box 蛋白 CORONATINE INSENSITIVE 1a（COI1a）的相互作用，与 ZINC-FINGER PROTEIN EXPRESSED IN INFLORESCENCE MERISTEM（ZIM1）相互作用，并增加了其稳定性，后者是茉莉酸（JA）信号通路的一个核心组成部分。LG1 启动子的自然变异与近交系之间的抗叶蝉性有关。此外，双子叶植物拟南芥（Arabidopsis thaliana）中与 LG1 相关的基因 SPL8 的功能丧失突变赋予了抗叶蝉性。本研究揭示了 lg1 的抗虫机制，为培育抗虫作物提供了理论依据和种质资源。
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