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P 体成分 DECAPPING5 和花抑制因子 SISTER OF FCA 调控拟南芥 FLOWERING LOCUS C 的转录。

The P-body component DECAPPING5 and the floral repressor SISTER OF FCA regulate FLOWERING LOCUS C transcription in Arabidopsis.

机构信息

The National Engineering Lab of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.

出版信息

Plant Cell. 2023 Sep 1;35(9):3303-3324. doi: 10.1093/plcell/koad151.

DOI:10.1093/plcell/koad151
PMID:37220754
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10473201/
Abstract

Flowering is the transition from vegetative to reproductive growth and is critical for plant adaptation and reproduction. FLOWERING LOCUS C (FLC) plays a central role in flowering time control, and dissecting its regulation mechanism provides essential information for crop improvement. Here, we report that DECAPPING5 (DCP5), a component of processing bodies (P-bodies), regulates FLC transcription and flowering time in Arabidopsis (Arabidopsis thaliana). DCP5 and its interacting partner SISTER OF FCA (SSF) undergo liquid-liquid phase separation (LLPS) that is mediated by their prion-like domains (PrDs). Enhancing or attenuating the LLPS of both proteins using transgenic methods greatly affects their ability to regulate FLC and flowering time. DCP5 regulates FLC transcription by modulating RNA polymerase II enrichment at the FLC locus. DCP5 requires SSF for FLC regulation, and loss of SSF or its PrD disrupts DCP5 function. Our results reveal that DCP5 interacts with SSF, and the nuclear DCP5-SSF complex regulates FLC expression at the transcriptional level.

摘要

开花是植物从营养生长向生殖生长的转变，对植物的适应和繁殖至关重要。开花时间调控的核心因子是开花关键基因 FLC，解析其调控机制可为作物改良提供重要信息。在这里，我们报道了去帽蛋白 5（DCP5）作为处理体（P 体）的一个组成部分，调节拟南芥（Arabidopsis thaliana）中 FLC 的转录和开花时间。DCP5 及其相互作用伙伴 FCA 姐妹蛋白（SSF）通过其类朊病毒结构域（PrD）发生液-液相分离（LLPS）。使用转基因方法增强或减弱这两种蛋白质的 LLPS 会极大地影响它们调节 FLC 和开花时间的能力。DCP5 通过调节 RNA 聚合酶 II 在 FLC 基因座上的富集来调节 FLC 的转录。DCP5 需要 SSF 来调节 FLC，而 SSF 或其 PrD 的缺失会破坏 DCP5 的功能。我们的结果表明，DCP5 与 SSF 相互作用，核 DCP5-SSF 复合物在转录水平上调节 FLC 的表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73e8/10473201/d359c71e830b/koad151f10.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73e8/10473201/26be13da67f3/koad151f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73e8/10473201/767f381e3672/koad151f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73e8/10473201/0f5a5eec24f9/koad151f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73e8/10473201/45f324653928/koad151f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73e8/10473201/f9849f9630c0/koad151f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73e8/10473201/d359c71e830b/koad151f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73e8/10473201/94e6324bd53e/koad151f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73e8/10473201/036ec959bc5f/koad151f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73e8/10473201/58830bdf96fd/koad151f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73e8/10473201/e018187d0a6e/koad151f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73e8/10473201/26be13da67f3/koad151f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73e8/10473201/767f381e3672/koad151f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73e8/10473201/0f5a5eec24f9/koad151f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73e8/10473201/45f324653928/koad151f8.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73e8/10473201/d359c71e830b/koad151f10.jpg

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