Kovács K L, Tigyi G, Alfonz H
Prep Biochem. 1985;15(5):321-34. doi: 10.1080/00327488508062449.
Hydrogenase was purified from the photosynthetic bacterium Thiocapsa roseopersicina to homogeneity by various methods. Conventional techniques included separation of the crude protein extract on Phenyl-Sepharose CL 4B, DEAE-cellulose DE52, and chromatofocusing columns or on preparative polyacrylamide gel-electrophoresis. The same protein was isolated by fast protein liquid chromatography (FPLC) in two steps. Comparison of the two different approaches clearly show the superiority of the FPLC method both in enzyme recovery yield and in time requirement.
通过多种方法从光合细菌玫瑰色硫囊菌中纯化氢化酶至同质状态。传统技术包括在苯基琼脂糖凝胶CL 4B、DEAE-纤维素DE52和色谱聚焦柱上或在制备性聚丙烯酰胺凝胶电泳上分离粗蛋白提取物。通过快速蛋白质液相色谱（FPLC）分两步分离得到相同的蛋白质。两种不同方法的比较清楚地表明了FPLC方法在酶回收率和时间需求方面的优越性。
