School of Biological Science and Medical Engineering, State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China; National Demonstration Center for Experimental Biomedical Engineering Education, Southeast University, Nanjing 210096, China; Collaborative Innovation Center of Suzhou Nano Science and Technology, Suzhou 215123, China.
School of Biological Science and Medical Engineering, State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China; National Demonstration Center for Experimental Biomedical Engineering Education, Southeast University, Nanjing 210096, China; Collaborative Innovation Center of Suzhou Nano Science and Technology, Suzhou 215123, China.
Food Chem. 2024 Dec 15;461:140794. doi: 10.1016/j.foodchem.2024.140794. Epub 2024 Aug 8.
This study aimed to prepare soy protein isolate-xanthan gum complexes (SPI-XG) at pH 7.0 and as emulsifiers to prepare Pickering emulsions for delivering quercetin (Que). The results showed that SPI-XG exhibited a gel network structure in which protein particles were embedded. Fourier transform infrared spectroscopy (FTIR) and molecular docking elucidated that SPI-XG formed through hydrogen bonding, hydrophobic, and electrostatic interactions. Three-phase contact angle (θo/w) of SPI-XG approached 90° with biphasic wettability. SPI-XG adsorbed at the oil-water interface to form an interfacial layer with a gel network structure, which prevented droplet aggregation. Following in vitro simulated digestion, Que displayed higher bioaccessibility in SPI-XG stabilized Pickering emulsions (SPI-XG PEs) than SPI stabilized Pickering emulsions. In conclusion, SPI-XG PEs were a promising system for Que delivery.
本研究旨在制备 pH 值为 7.0 的大豆分离蛋白-黄原胶复合物（SPI-XG），并将其作为乳化剂用于制备载运槲皮素（Que）的 Pickering 乳液。结果表明，SPI-XG 呈现出一种蛋白质颗粒嵌入其中的凝胶网络结构。傅里叶变换红外光谱（FTIR）和分子对接表明，SPI-XG 是通过氢键、疏水相互作用和静电相互作用形成的。SPI-XG 的三相接触角（θo/w）接近 90°，具有双相润湿性。SPI-XG 吸附在油水界面上，形成具有凝胶网络结构的界面层，阻止液滴聚集。经过体外模拟消化，SPI-XG 稳定的 Pickering 乳液（SPI-XG PE）中 Que 的生物利用度高于 SPI 稳定的 Pickering 乳液。综上所述，SPI-XG PE 是 Que 递送的一种有前途的系统。
