College of Food Science, Northeast Agricultural University, Harbin, China.
Heilongjiang Green Food Science Research Institute, Harbin, China.
J Sci Food Agric. 2022 Nov;102(14):6273-6284. doi: 10.1002/jsfa.11976. Epub 2022 May 26.
Protein-polysaccharide complexes have been widely used to stabilize high-internal-phase emulsion (HIPEs). However, it is still unknown whether soy protein isolate-dextran (SPI-Dex) complexes can stabilize HIPEs or what is the effect of Dex concentration on the HIPEs. Furthermore, the non-covalent interaction mechanism between SPI and Dex is also unclear. Therefore, we fabricated SPI-Dex complexes and used them to stabilize HIPEs-loaded quercetin and explore the interaction mechanism between SPI and Dex, as well as the effect of Dex concentration on the particle size, ζ-potential, microstructure, rheology, quercetin encapsulation efficiency, and gastrointestinal fate of the HIPEs.
Spectral analysis (fourier transform infrared spectroscopy, ultraviolet spectroscopy, and fluorescence spectroscopy) results identified the formation of SPI-Dex complexes, and indicated that the addition of Dex changed the spatial structure of SPI, whereas thermodynamic analysis (ΔH > 0, ΔS > 0) showed that hydrophobic interactions were the main driving forces in the formation of SPI-Dex complexes. Compared with HIPEs stabilized by SPI, the SPI-Dex complex-stabilized HIPEs had smaller particles (3000.33 ± 201.22 nm), as well as higher ζ-potential (-21.73 ± 1.10 mV), apparent viscosities, modulus, and quercetin encapsulation efficiency (98.19 ± 0.14%). In addition, in vitro digestion revealed that SPI-Dex complex-stabilized HIPEs significantly reduced the release of free fatty acid and improved quercetin bioaccessibility.
HIPEs stabilized by SPI-Dex complexes delayed the release of free fat acid and improved the bioaccessibility of quercetin, and may be help in designing delivery systems for bioactive substances with specific properties. © 2022 Society of Chemical Industry.
蛋白质-多糖复合物已广泛用于稳定高内相比乳液（HIPE）。然而，目前尚不清楚大豆分离蛋白-葡聚糖（SPI-Dex）复合物是否可以稳定 HIPE，或者 Dex 浓度对 HIPE 的影响如何。此外，SPI 和 Dex 之间的非共价相互作用机制也不清楚。因此，我们制备了 SPI-Dex 复合物，并将其用于稳定负载槲皮素的 HIPE，以探索 SPI 和 Dex 之间的相互作用机制，以及 Dex 浓度对 HIPE 粒径、ζ-电位、微观结构、流变学、槲皮素包封效率和胃肠道命运的影响。
光谱分析（傅里叶变换红外光谱、紫外光谱和荧光光谱）结果证实了 SPI-Dex 复合物的形成，并表明 Dex 的加入改变了 SPI 的空间结构，而热力学分析（ΔH＞0，ΔS＞0）表明疏水相互作用是形成 SPI-Dex 复合物的主要驱动力。与由 SPI 稳定的 HIPE 相比，由 SPI-Dex 复合物稳定的 HIPE 具有更小的颗粒（3000.33±201.22nm）、更高的 ζ-电位（-21.73±1.10mV）、表观粘度、模量和槲皮素包封效率（98.19±0.14%）。此外，体外消化表明，SPI-Dex 复合物稳定的 HIPE 可显著减少游离脂肪酸的释放并提高槲皮素的生物利用度。
由 SPI-Dex 复合物稳定的 HIPE 可延迟游离脂肪酸的释放并提高槲皮素的生物利用度，可能有助于设计具有特定性质的生物活性物质的递送系统。© 2022 化学工业协会。
