College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; College of JunCao Science and Ecology (College of Carbon Neutrality), Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Bioresour Technol. 2024 Oct;410:131272. doi: 10.1016/j.biortech.2024.131272. Epub 2024 Aug 13.
Hybrid Pennisetum, a top biomass energy source, faces usage limitations because of its scarce lactic acid bacteria and high fiber content. This study assessed the influence of rumen fluid pretreatment on hybrid Pennisetum's silage, with focus on silage duration and rumen fluid effects on quality and fiber decomposition. Advanced third-generation sequencing was used to track microbial diversity changes and revealed that rumen fluid considerably enhanced dry matter, crude protein, and water-soluble carbohydrates, thus improving fermentation quality to satisfactory pH levels (3.40-3.67). Ideal results, including the highest fiber breakdown and enzymatic efficiency (47.23 %), were obtained with 5 % rumen fluid in 60 days. The addition of rumen fluid changed the dominant species, including Paucilactobacillus vaccinostercus (0.00 % vs. 18.21 %) and Lactiplantibacillus plantarum (21.03 % vs. 47.02 %), and no Enterobacter was detected in the high-concentration treatments. Moreover, strong correlations were found between specific lactic acid bacteria and fermentation indicators, revealing the potential of achieving efficient and economically beneficial hybrid Pennisetum production.
杂交狼尾草是一种重要的生物量能源，但由于其乳酸菌含量少、纤维含量高，应用受到限制。本研究评估了瘤胃液预处理对杂交狼尾草青贮的影响，重点关注青贮时间以及瘤胃液对青贮品质和纤维分解的影响。先进的第三代测序技术用于跟踪微生物多样性的变化，结果表明瘤胃液显著提高了干物质、粗蛋白和水溶性碳水化合物的含量，从而改善了发酵品质，使 pH 值达到理想水平（3.40-3.67）。在 60 天内，添加 5%的瘤胃液可获得最佳的纤维分解和酶效（47.23%）。瘤胃液的添加改变了优势种，包括少乳酸杆菌（0.00%对 18.21%）和植物乳杆菌（21.03%对 47.02%），且高浓度处理中未检出肠杆菌。此外，特定乳酸菌与发酵指标之间存在很强的相关性，这表明实现高效和经济上有益的杂交狼尾草生产是有潜力的。
